What are the differences between Recombinant Human IL-6 Protein (Catalog # 206-IL) and Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL)?

Name: Recombinant Human IL-6 (HEK293-expressed) Protein
Brand: R&D Systems
SKU: 7270-IL
Rating: 5 (3 reviews)

Recombinant Human IL-6 Protein (Catalog # 206-IL) is raised in E. coli. The Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL) is raised in HEK293. Because #7270-IL is raised in mammalian cells, it is expected to have post translational modifications that are absent in the E. coli-derived version (206-IL). The HEK293-derived #7270-IL exhibits greater activity than the E. coli-derived #206-IL in the same activity assay.

Recombinant Human IL-6 (HEK293-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7270-IL

Citations (5)

(3)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	P05231.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-6 (HEK293-expressed) Protein

Product Specifications for Recombinant Human IL-6 (HEK293-expressed) Protein

Source	Human embryonic kidney cell, HEK293-derived human IL-6 protein Val30-Met212
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala28 & Val30
Predicted Molecular Mass	20.8 kDa
SDS-PAGE	22 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED₅₀ for this effect is 0.1-0.6 ng/mL.

Scientific Data Examples for Recombinant Human IL-6 (HEK293-expressed) Protein

	Recombinant Human IL-6 (HEK293-expressed) Protein Bioactivity Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. The ED₅₀ for this effect is 0.1-0.6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7270-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7270-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6 (6). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 13). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 14).

Item	Value
References	Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249. Schuett, H. et al. (2009) Thromb. Haemost. 102:215. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143. Hirano, T. et al. (1986) Nature 324:73. Kestler, D.P. et al. (1995) Blood 86:4559. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48. Alberti, L. et al. (2005) Cancer Res. 65:2. Murakami, M. et al. (1993) Science 260:1808. Muller-Newen, G. (2003) Sci. STKE 2003:PE40. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125. Cerutti, A. et al. (1998) J. Immunol. 160:2145.
Long Name	Interleukin 6
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names	B-cell differentiation factor, B-cell stimulatory factor 2, BSF-2, BSF2, CDF, CTL differentiation factor , HSF, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, MGI-2A, hybridoma growth factor, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6

Citations for Recombinant Human IL-6 (HEK293-expressed) Protein (5)

Showing 1 - 5 of 5 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-6 (HEK293-expressed) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AK Ajay et al. (2022), Deletion of STAT3 from Foxd1 cell population protects mice from kidney fibrosis by inhibiting pericytes trans-differentiation and migration Cell Reports, 38(10):110473.
PMID: 35263586
Species: Xenografts
Sample Types: Whole Cells
Applications: Bioassay

T Manshouri et al. (2022), GLI1 activates pro-fibrotic pathways in myelofibrosis fibrocytes Cell Death Disease, 2022;13(5):481.
PMID: 35595725
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Cai et al. (2022), Kynurenine metabolites predict survival in pulmonary arterial hypertension: A role for IL-6/IL-6Ralpha Scientific Reports, 12(1):12326.
PMID: 35853948
Species: Human
Sample Types: Protein
Applications: ELISA Capture

S Jadhav et al. (2021), A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma PLoS ONE, 16(1):e0245614.
PMID: 33481901
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Y Wada et al. (2020), The inhibitory effect of TU-100 on hepatic stellate cell activation in the tumor microenvironment Oncotarget, 11(49):4593-4604.
PMID: 33346211

There are no citations that match your criteria.

Showing 1 - 5 of 5 citations Showing All

Customer Reviews for Recombinant Human IL-6 (HEK293-expressed) Protein (3)

4.7 out of 5

3 customer ratings

5 stars

67%

4 stars

33%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 3 of 3 reviews Showing All

Filter By:

Recombinant Human IL-6 (HEK293-expressed) Protein

Name: Anonymous

Reason for Rating: Got a strong band in WB.

Verified Customer | Posted 03/17/2023

Western blot analysis of IL-6 expression in HEK293 whole cell lysate.
Recombinant Human IL-6 (HEK293-expressed) Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Reason for Rating: Activation of HEK Blue Cells with EC50 of 0.2ng/ml as stated in datasheet.

Verified Customer | Posted 04/15/2021

HEK Blue IL-6 assay from Invivogen
Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 07/27/2018

There are no reviews that match your criteria.

Showing 1 - 3 of 3 reviews Showing All

FAQs for Recombinant Human IL-6 (HEK293-expressed) Protein

What are the differences between Recombinant Human IL-6 Protein (Catalog # 206-IL) and Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL)?

Recombinant Human IL-6 Protein (Catalog # 206-IL) is raised in E. coli. The Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL) is raised in HEK293. Because #7270-IL is raised in mammalian cells, it is expected to have post translational modifications that are absent in the E. coli-derived version (206-IL). The HEK293-derived #7270-IL exhibits greater activity than the E. coli-derived #206-IL in the same activity assay.

Recombinant Human IL-6 (HEK293-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Human IL-6 (HEK293-expressed) Protein

Scientific Data Examples for Recombinant Human IL-6 (HEK293-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

7270-IL

Carrier

7270-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-6

Citations for Recombinant Human IL-6 (HEK293-expressed) Protein (5)

Customer Reviews for Recombinant Human IL-6 (HEK293-expressed) Protein (3)

Customer Images

Recombinant Human IL-6 (HEK293-expressed) Protein

Recombinant Human IL-6 (HEK293-expressed) Protein

FAQs for Recombinant Human IL-6 (HEK293-expressed) Protein

Product Documents for Recombinant Human IL-6 (HEK293-expressed) Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected