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Recombinant Human Glypican 3 Protein Best Seller

Catalog # 2119-GP | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
2119-GP-050/CF

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With Carrier
2119-GP-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Glypican 3 is subject to endoproteolytic processing by proprotein convertases (PC). By amino acid sequencing, three peptides (the first with a blocked N-terminus most likely starts with Gln25, the second peptide starts with Ser359 after a furin cleavage site, and the third peptide starts with Val483) are present in the recombinant GPC3 preparation. Peptides 2 and 3 are detected at a 1:1 ratio. All three peptides remained associated via disulfide bonds.

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Glypican 3 protein
Gln25-His559 with a C-terminal 6-His tag

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln25 predicted, Ser359 & Val483

Predicted Molecular Mass

61.6 kDa

SDS-PAGE

60-100 kDa, non-reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Glypican 3 is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Recombinant Human FGF basic 146 aa (Catalog # 233-FB) that produces 50% of the optimal binding response is approximately 0.6-3 ng/mL.

Scientific Data Images for Recombinant Human Glypican 3 Protein

Graph showing bioactivity of human Glypican-3 protein

Bioactivity of Human Glypican-3

Recombinant human Glypican 3 (2119-GP) binds recombinant human FGF basic 146 in a functional ELISA. The concentration of recombinant human FGF basic 146 that produces 50% of the optimal binding response is 0.6-3 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2119-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2119-GP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 3

Glypicans (GPC) are a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. While glypican proteins do not share strong amino acid sequence identity (they range from 17-63%), the conserved cysteine residues in their CRDs suggests similarity in their
three‑dimensional structure (1, 2).

Mutations in GPC3 cause a rare disorder in humans, Simpson-Golabi-Behmel Syndrome, which is characterized by pre and postnatal overgrowth of multiple tissues and organs and an increased risk for developing embryonic tumors (3). These features are also present in the mouse knock-out of GPC3 indicating that GPC3 regulates cell survival and inhibits cell proliferation during development (4). Glypican 3 has been implicated in regulating many different signaling pathways including: IGF, FGF, BMP and Wnt. An endoproteolytic processing of GPC3 by proprotein convertases is required for the modulation of Wnt signaling (5). Direct interaction with FGF-basic has been observed and is mediated by the heparan sulfate chains (6).

References

  1. Filmus, J. and S.B. Selleck (2001) J. Clinical Invest. 108:497. 
  2. De Cat, B and G. David (2001) Seminars in Cell & Dev. Biol. 12:117. 
  3. Pilia, G. et al. (1996) Nat. Genet. 12: 241. 
  4. Cano-Gauci, D.F. et al. (1999) J. Cell Biol. 146: 255.
  5. De Cat, B. et al. (2003) J. Cell Biol. 163:625.
  6. Song, H.H. et al. (1997) J. Biol. Chem. 272:7574.

Alternate Names

GPC3

Entrez Gene IDs

2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)

Gene Symbol

GPC3

UniProt

Product Documents for Recombinant Human Glypican 3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Glypican 3 Protein

For research use only

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