Recombinant Protein A/G/L Protein

Name: Recombinant Protein A/G/L Protein
Brand: Novus Biologicals
SKU: NBP2-34985

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34985

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Protein A/G/L Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 805 amino acids corresponding to Protein A/G/L Source: E. coli

Amino Acid Sequence: NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEE PRARPGSGSG KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAG

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Protein A/G/L as determined by LAL method.

Predicted Molecular Mass

89.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Protein A/G/L is a genetically engineered protein containing 5 IgG-binding regions of protein A, 2 of protein G and 5 of Protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G/L to ensure the maximum specific IgG binding. The recombinant Protein A/G/L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A/G/L binds to human, mouse, rat cow, goat, sheep, rabbit, guinea pig, pig, dog and cat IgG.

Specificity

Protein A/G/L binds to human, mouse, rat cow, goat, sheep, rabbit, guinea pig, pig, dog and cat IgG.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Protein A/G/L Protein

SDS-PAGE: Recombinant Protein A/G/L Protein [NBP2-34985]

SDS-Page: Protein A/G/L Protein [NBP2-34985]

Protein A/G/L Protein [NBP2-34985]

HPLC: Recombinant Protein A/G/L Protein [NBP2-34985]

HPLC: Recombinant Protein A/G/L Protein [NBP2-34985] - Protein A/G/L Protein [NBP2-34985]

Formulation, Preparation and Storage

NBP2-34985

Formulation	Lyophilized from additive free solution.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Reconstitute with distilled water or saline.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Protein A/G/L

Protein A/G/L is a genetically engineered protein that combines the IgG binding profiles of all Protein A, Protein G and Protein L. It is a gene fusion product. Recombinant fusion protein A/G/L contains five Ig-binding regions of protein L, five IgG binding domains from Protein A, and two Ig-binding region of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding.

Additional Protein A/G/L Products

Product Documents for Recombinant Protein A/G/L Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Protein A/G/L Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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