Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF

Name: Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 2406-BR
Price: 493 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 2406-BR

Citations (1)

Product Datasheet / COA / SDS

Catalog #

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	NP_004320
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF

Product Specifications for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human BMPR-IA/ALK-3 protein

Human BMPR-IA (Met1-Arg152) Accession # NP_004320	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gln24 predicted: No results obtained, sequencing might be blocked

Predicted Molecular Mass

40.8 kDa (monomer)

SDS-PAGE

59 kDa, reducing conditions

Activity

Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 0.03-0.12 μg/mL in the presence of 15 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2406-BR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMPR-IA/ALK-3

Bone Morphogenetic Protein Receptor IA (BMPR‑IA), also known as ALK‑3, BRK‑1, and CD292, is a glycosylated 60‑65 kDa type I receptor in the TGF‑ beta serine/threonine kinase receptor family (1‑3). Binding of TGF‑ beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Mature human BMPR‑IA consisits of a 129 amino acid (aa) extracellular domain (ECD), a 24 aa transmembrane segment, and a 356 aa cytoplasmic region that contains the tyrosine kinase domain (4). Within the ECD, human BMPR-IA shares 98% and 97% aa sequence identity with mouse and rat BMPR‑IA, respectively. BMPR‑IA is involved in the development and function of a wide range of tissues. During early embryogenesis it is required for migration of the anterior visceral endoderm (AVE) and proper development of the anterior-posterior axis (5). Tissue‑specific conditional knockout experiments have demonstrated the importance of BMPR‑IA in the development and morphogenesis of the heart, lung, palate, teeth, and mandible (6‑8). In the adult, BMPR‑IA plays a role in glucose‑stimulated insulin secretion by pancreatic beta cells, osteoclast activity and bone remodeling, reactive astrocyte‑mediated scar formation following spinal cord injury, and ovulation and fertility (9‑12).

Item	Value
References	Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1. ten Dijke, P. et al. (1993) Oncogene 8:2879. Miura, S. et al. (2010) Dev. Biol. 341:246. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878. Sun, J. et al. (2008) Am. J. Pathol. 172:571. Li, L. et al. (2011) Dev. Biol. 349:451. Goulley, J. et al. (2007) Cell Metab. 5:207. Kamiya, N. et al. (2008) J. Bone Miner. Res. 23:2007. Sahni, V. et al. (2010) J. Neurosci. 30:1839. Edson, M.A. et al. (2010) Mol. Endocrinol. 24:1251.
Long Name	Bone Morphogenetic Protein Receptor IA/Activin Receptor-like Kinase 3
Entrez Gene IDs	657 (Human); 12166 (Mouse)
Alternate Names	ACVRLK310q23del, ALK-3, ALK3EC 2.7.11.30, BMP type-1A receptor, BMPR-1A, BMPR-IA, BMPR1A, BMPRIA, CD292, CD292 antigen, EC 2.7.11, SKR5, Serine/threonine-protein kinase receptor R5, bone morphogenetic protein receptor type-1A, bone morphogenetic protein receptor, type IA, type II-like kinase 3

Citations for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Cell Culture Supernates, Recombinant Protein
Applications: Surface Plasmon Resonance

T Mang et al. (2020-01-01), BMPR1A is necessary for chondrogenesis and osteogenesis while BMPR1B prevents hypertrophic differentiation J. Cell. Sci., 0(0).
PMID: 32764110

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Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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