Recombinant Human B7-2/CD86 Fc Chimera Protein, CF

Name: Recombinant Human B7-2/CD86 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 141-B2

Catalog # 141-B2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (5)

Product Datasheet / COA / SDS

A New rh B7-2 is Available! It has higher purity, ~3 fold better activity, and is HEK293 expressed!

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	AAB03814
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human B7-2/CD86 Fc Chimera Protein, CF

Technical Data
Citations (5) / Reviews

Product Specifications for Recombinant Human B7-2/CD86 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human B7-2/CD86 protein

Human B7-2 (Leu20-His239) Accession # AAB03814	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu20

Predicted Molecular Mass

52.7 kDa (monomer)

SDS-PAGE

52.7 kDa (monomer)

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED₅₀ for this effect is 0.2‑0.8 µg/mL in the presence of PHA.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

141-B2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: B7-2/CD86

B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance (1, 2). Mature human B7-2 consists of a 224 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 61 aa cytoplasmic tail (3, 4). Within the ECD, human B7-2 shares 59% aa sequence identity with mouse and rat B7-2. Alternative splicing of human B7-2 generates additional isoforms that lack both Ig-like domains or a region that includes the transmembrane segment. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells, and monocytes (4-7), as well as on vascular endothelial cells (8). B7-2 and the closely related B7-1/CD80 exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent co-stimulation (3-5, 7, 9-11). B7-1 and B7-2 additionally bind the CD28-related protein, CTLA-4, which is up‑regulated and recruited to the immunological synapse (IS) at the onset of T cell activation (3-5, 7, 9, 10). CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function (12). B7-2 is expressed earlier than B7-1 following APC activation (6), and both proteins bind with higher affinity to CTLA-4 than to CD28 (10). B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS (13). The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response (14). Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses (15).

Item	Value
References	Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180. Freeman, G.J. et al. (1993) Science 262:909. Azuma, M. et al. (1993) Nature 366:76. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185. Lenschow, D.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11054. Hathcock, K.S. et al. (1993) Science 262:905. Seino, K. et al. (1995) Int. Immunol. 7:1331. Chen, C. et al. (1994) J. Immunol. 152:4929. Lanier, L.L. et al. (1995) J. Immunol. 154:97. Rudd, C.E. et al. (2009) Immunol. Rev. 229:12. Wing, K. et al. (2011) Trends Immunol. 32:428. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401. Kuchroo, V.K. et al. (1995) Cell 80:707. Short, J.J. et al. (2006) Virus Res. 122:144.
Entrez Gene IDs	942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)
Alternate Names	Activation B7-2 antigen, B-lymphocyte activation antigen B7-2, B7-2, B7-2 antigen, B70, B72, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86, CD86 antigen, CD86 molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86

Citations for Recombinant Human B7-2/CD86 Fc Chimera Protein, CF (5)

Showing 1 - 5 of 5 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human B7-2/CD86 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Popugailo et al. (2023-06-28), The homodimer interfaces of costimulatory receptors B7 and CD28 control their engagement and pro-inflammatory signaling Journal of biomedical science
PMID: 37381064
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

H Sic et al. (2017-12-15), An Activating Janus Kinase-3 Mutation Is Associated with Cytotoxic T Lymphocyte Antigen-4-Dependent Immune Dysregulation Syndrome Front Immunol, 2017-18(0):1824.
PMID: 29375547
Sample Types: Protein
Applications: Bioassay

Lutz Jermutus (2016-11-23), A CD80-Biased CTLA4-Ig Fusion Protein with Superior In Vivo Efficacy by Simultaneous Engineering of Affinity, Selectivity, Stability, and FcRn Binding J. Immunol., 2016-10(0).
PMID: 27881707
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Choi J et al. (2015-07-20), Genomic landscape of cutaneous T cell lymphoma. Nat Genet, 2015-047(9):1011-9.
PMID: 26192916
Sample Types: N/A
Applications: ELISA (Standard)

Sakthivel P et al. (2007-04-01), The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 2007-0261(4):399-407.
PMID: 17391115

There are no citations that match your criteria.

Showing 1 - 5 of 5 citations Showing All

Product Documents for Recombinant Human B7-2/CD86 Fc Chimera Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS