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Recombinant HCoV-OC43 Spike His-tag Protein, CF

Catalog # 10989-CV | R&D Systems, Inc. a Bio-Techne Brand
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10989-CV-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived hcov-oc43 Spike protein
Val15-Pro1297 (Arg754Ser, Arg757Ser), with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val15

Predicted Molecular Mass

144 kDa

SDS-PAGE

>150 kDa, under reducing conditions.

Activity

Measured by its ability to agglutinate mouse red blood cells. The ED50 for this effect is 0.10‑1.00 µg/mL.

Scientific Data Images for Recombinant HCoV-OC43 Spike His-tag Protein, CF

Recombinant HCoV-OC43 Spike His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant HCoV-OC43 Spike His-tag Protein (Catalog # 10989-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at >150 kDa.

Formulation, Preparation and Storage

10989-CV
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Spike

HCoV-OC43, a virus first isolated in 1960's that accounts for ~ 20% of the common cold, belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-229E, HCoV-HKU1 and HCov-NL63, plus three viruses that cause the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (3, 4). The CoV-OC43 N protein is a ~50 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (5). The CoV‑OC43 N protein shares 64% amino acid sequence identity with CoV-HKU1 N protein. the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity. Cross activity of antibodies among different strains should be rigorously tested when designing serological diagnostic kits (6, 7).

References

  1. St-Jean, J.R. et al. (2004) J. Virol. 78:8824.
  2. Vabret, A. et al. (2003) Fr Clin Infect Dis. 36:985.
  3. Chang, C.K. et al. (2006) J. Biomed. Sci. 13:59.
  4. Hurst, K.R. et al. (2009) J. Virol. 83:7221.
  5. Huang, C.Y. et al. (2009) Protein Sci. 18:2209.
  6. Chan, K.H. et al. (2005) Clin Diagn Lab Immunol. 12:1317.
  7. Mourez, T. et al. (2007) J. Virol Methods. 139:175.

Long Name

Spike Protein

Alternate Names

S Protein

Entrez Gene IDs

918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

Additional Spike Products

Product Documents for Recombinant HCoV-OC43 Spike His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant HCoV-OC43 Spike His-tag Protein, CF

For research use only

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