Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

Name: Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF
Brand: R&D Systems
SKU: 10605-CV

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10605-CV

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Key Product Details

Source:	HEK293
Accession #:	YP_003767.1
Applications:	Bioactivity

View Full Technical Details for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

Product Specifications for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

Source	Human embryonic kidney cell, HEK293-derived hcov-nl63 Spike RBD protein Ala475-Asp634, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala475 & Leu476
Predicted Molecular Mass	19 kDa
SDS-PAGE	30-38 kDa, under reducing conditions
Activity	Recombinant HCoV-NL63 Spike RBD His-tag Protein (Catalog # 10605-CV) binds Recombinant Human ACE-2 Fc Chimera Protein (Catalog # 10544-ZN) in a functional ELISA.

Scientific Data Examples for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

	Recombinant HCoV-NL63 Spike RBD His-tag Protein Binding Activity. Recombinant HCoV-NL63 Spike RBD His-tag Protein (Catalog # 10605-CV) binds Recombinant Human ACE-2 Fc Chimera Protein (10544-ZN) in a functional ELISA.
	Recombinant HCoV-NL63 Spike RBD His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant HCoV-NL63 Spike RBD His-tag (Catalog # 10605-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-38 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10605-CV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Spike RBD

HCoV-NL63, a virus first isolated from a child suffering from respiratory disease in 2003, belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-229E, HCoV-HKU1 and HCov-OC43, plus three viruses that cause the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2). HCov-NL63 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. As with most coronaviruses, proteolytic cleavage of the S protein generates two distinct peptides, S1 and S2 subunits. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion. Although HCoV-NL63 S protein shares high homology (56%) with HCoV-229E, it does not employ CD13 (aminopeptidase N) as the receptor like HCoV-229E. Instead, HCoV-NL63 engages Angiotensin-Converting Enzyme 2 (ACE-2), the same receptor as SARS-CoV and SARS-CoV2, for cellular entry and replication (3). The receptor binding domain (RBD) of HCoV-NL63 is located at C-terminal region of S1 subunit (4, 5). Although NL63-CoV and SARS-CoV do not share structural homology in RBD region, they bind an overlapping region of ACE-2 (6, 7).

Item	Value
References	Van der Hoek, L. et al. (2004) Nat. Med. 10:368. Fouchier, R.M. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:6212. Hofmann, H. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:7988. Hofmann, H. et al. (2006) J. Virol. 80:8639. Lin, H. et al. (2008) J. Gen. Virol. 89:1015. Li, W. et al. (2007) Virology 367:367. Wu, K. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:19970.
Long Name	Spike Receptor Binding Domain
Entrez Gene IDs	3200426 (HCoV-HKU1); 14254594 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)
Alternate Names	Spike RBD

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Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

Scientific Data Examples for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Spike RBD

Citations for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

Product Documents for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

Product Datasheets

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