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Recombinant HCoV-HKU1 Spike RBD His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10600-CV

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10600-CV-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived hcov-hku1 Spike RBD protein
Thr310-Tyr624, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr310

Predicted Molecular Mass

36 kDa

SDS-PAGE

55-65 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant HCoV-HKU1 Spike RBD His-tag Protein, CF

SDS-PAGE gel of purified recombinant HCoV-HKU1 Spike RBD protein with His-tag

Recombinant HCoV-HKU1 Spike RBD His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant HCoV-HKU1 Spike RBD His-tag (Catalog # 10600-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-65 kDa.

Formulation, Preparation and Storage

10600-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike RBD

HCoV-HKU1 was identified in Hong Kong in 2005 as a new human coronavirus (1). Coronaviruses are a family of viruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein(N). There are two well-known human coronavirus families that infect humans: Alpha coronaviruses which includes HCoV-229E and HCoV-NL63; beta coronaviruses that includes HCov-OC43, Severe Acute Respiratory Syndrome (SARS-CoV), Middle East Respirator Syndrome (MERS-CoV), and global pandemic Covid-19 (SARS-CoV2) (2). The HCoV-HKU1 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. As with most coronaviruses, proteolytic cleavage of the S protein generates two distinct peptides, S1 and S2 subunits. The S1 subunit is focused on attachment of the protein to the host receptor, while the S2 subunit is involved with cell fusion. The receptor binding domain (RBD) of HCoV-HKU1 is located at C‑terminal region of S1 subunit, similar to SARS‑COV, MERS‑COV and SARS‑COV2, but the RBD regions do not share significant amino acid sequence identity (3). HCoV‑HKU1 has been demonstrated to bind specifically to 9‑O‑acetylated sialic acids (9-O-Ac-Sias) attached as terminal residues to glycan chains on glycoproteins and lipids, but additional receptors remain unknown (4). HCoV‑HKU1, along with HCov-OC43, differ from other cornonaviruses in that their virions possess two types of surface projections, both involved in attachment: large "spikes" that are comprised of homotrimers of the S protein, and unique, smaller protrusions, comprised of the homodimeric hemagglutinin‑esterase (HE) (5).

References

  1. Woo, P. et al. (2005) J. Virol.79:884.
  2. Ogimi, C. et al. (2020) J Pediatric Infect Dis Soc doi: 10.1093/jpids/piaa037.
  3. Qian, Z. et al. (2015) J. Virol. 89:8816.
  4. Huang, X. et al. (2015) J Virol 89:7202.
  5. Hulswit, R.J.G. et al. (2019) PNAS 116:2681.

Long Name

Spike Receptor Binding Domain

Entrez Gene IDs

3200426 (HCoV-HKU1); 14254594 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

Additional Spike RBD Products

Product Documents for Recombinant HCoV-HKU1 Spike RBD His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant HCoV-HKU1 Spike RBD His-tag Protein, CF

For research use only

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