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Recombinant Cynomolgus Monkey PCSK9 His-tag Protein, CF

Catalog # 10469-SE | R&D Systems, Inc. a Bio-Techne Brand
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10469-SE-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Mature form & pro domain

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Proprotein Convertase 9/PCSK9 protein
Gln31-Gln152 (pro domain) & Ser153-Gln692 (mature form) with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser153 (mature form) & Gln32 (pro domain)

Predicted Molecular Mass

14 kDa & 58 kDa

SDS-PAGE

19 kDa and 61 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LDLR His-tag  (Catalog # 2148-LD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Proprotein Convertase 9/PCSK9 His-tag (Catalog # 10469-SE) binds with an ED50 of 35-280 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey PCSK9 His-tag Protein, CF

Recombinant Cynomolgus Monkey PCSK9 His-tag Protein Binding Activity

Recombinant Cynomolgus Monkey PCSK9 His-tag Protein Binding Activity

When Recombinant Human LDLR (2148-LD) is immobilized at 1 μL/mL (100 μL/well), Recombinant Cynomolgus Monkey PCSK9 His-tag (10469-SE) binds with an ED50 of 35-280 ng/mL.
Recombinant Cynomolgus Monkey PCSK9 His-tag Protein SDS-PAGE

Recombinant Cynomolgus Monkey PCSK9 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Proprotein Convertase 9/PCSK9 His-tag (Catalog # 10469-SE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19 and 61 kDa under reducing conditions.

Formulation, Preparation and Storage

10469-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Proprotein Convertase 9/PCSK9

PCSK9 (proprotein convertase subtilisin kexin 9), also known as NARC-1, is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. It is highly expressed in the liver, intestine, and kidney and plays an important role in regulating LDL R expression and circulating cholesterol levels (1). PCSK9 is synthesized as precursor protein that is autocatalytically cleaved in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain (2). Within the secretion pathway, the prodomain remains associated with and functions as a chaperone for the catalytic domain (2). Although the other members of the proprotein convertase family demonstrate activity on several downstream targets, PCSK9 protease activity has only been demonstrated through its autocatalytic processing (3). PCSK9 plays a key role in the regulation of cholesterol metabolism by binding to hepatic LDL R, LRP-1, VLDL R, and Apolipoprotein E R2 and promoting their lysosomal degradation instead of recycling to the plasma membrane (4-7). It can also regulate cholesterol and triglyceride handling in the intestine and adipose tissue (8-10). These reported functions create significant interest in PCSK9 as a pharmacological target in cardiovascular disease (11). PCSK9 has been reported to interact with non-LDR targets as well, including mediators of inflammation and immunological processes (12).

References

  1. Schulz, R. et al. (2015) Basic Res. Cardiol. 110:4.
  2. Benjannet, S. et al. (2004) J. Biol. Chem. 279:48865.
  3. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
  4. Zhang, D.W. (2007) J. Biol. Chem. 282:18602.
  5. DeVay, R.M. et al. (2013) J. Biol. Chem. 288:10805.
  6. Canuel, M. et al. (2013) PLoS One 8:e64145.
  7. Poirier, S. et al. (2008) J. Biol. Chem. 283:2363.
  8. Levy, E. et al. (2013) Atherosclerosis 227:297.
  9. Le May, C. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:1484.
  10. Roubtsova, A. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:785.
  11. Chen, B. et. al. (2019) Curr.Top Med. Chem. 19:1790.
  12. Dixon, D.L. et. al. (2016) J. Clin. Lipidol. 10:1073.

Alternate Names

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9

Entrez Gene IDs

255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)

Gene Symbol

PCSK9

UniProt

Product Documents for Recombinant Cynomolgus Monkey PCSK9 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey PCSK9 His-tag Protein, CF

For research use only

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