Source	Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Proprotein Convertase 9/PCSK9 protein Gln31-Gln152 (pro domain) & Ser153-Gln692 (mature form) with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser153 (mature form) & Gln32 (pro domain)
Predicted Molecular Mass	14 kDa & 58 kDa
SDS-PAGE	19 kDa and 61 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human LDLR His-tag (Catalog # 2148-LD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Proprotein Convertase 9/PCSK9 His-tag (Catalog # 10469-SE) binds with an ED₅₀ of 35-280 ng/mL.

Recombinant Cynomolgus Monkey PCSK9 His-tag Protein, CF Scientific Data Examples

	Recombinant Cynomolgus Monkey PCSK9 His-tag Protein Binding Activity When Recombinant Human LDLR (2148-LD) is immobilized at 1 μL/mL (100 μL/well), Recombinant Cynomolgus Monkey PCSK9 His-tag (10469-SE) binds with an ED₅₀ of 35-280 ng/mL.
	Recombinant Cynomolgus Monkey PCSK9 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey Proprotein Convertase 9/PCSK9 His-tag (Catalog # 10469-SE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19 and 61 kDa under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10469-SE

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: Proprotein Convertase 9/PCSK9

PCSK9 (proprotein convertase subtilisin kexin 9), also known as NARC-1, is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. It is highly expressed in the liver, intestine, and kidney and plays an important role in regulating LDL R expression and circulating cholesterol levels (1). PCSK9 is synthesized as precursor protein that is autocatalytically cleaved in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain (2). Within the secretion pathway, the prodomain remains associated with and functions as a chaperone for the catalytic domain (2). Although the other members of the proprotein convertase family demonstrate activity on several downstream targets, PCSK9 protease activity has only been demonstrated through its autocatalytic processing (3). PCSK9 plays a key role in the regulation of cholesterol metabolism by binding to hepatic LDL R, LRP-1, VLDL R, and Apolipoprotein E R2 and promoting their lysosomal degradation instead of recycling to the plasma membrane (4-7). It can also regulate cholesterol and triglyceride handling in the intestine and adipose tissue (8-10). These reported functions create significant interest in PCSK9 as a pharmacological target in cardiovascular disease (11). PCSK9 has been reported to interact with non-LDR targets as well, including mediators of inflammation and immunological processes (12).

Item	Value
References	Schulz, R. et al. (2015) Basic Res. Cardiol. 110:4. Benjannet, S. et al. (2004) J. Biol. Chem. 279:48865. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55. Zhang, D.W. (2007) J. Biol. Chem. 282:18602. DeVay, R.M. et al. (2013) J. Biol. Chem. 288:10805. Canuel, M. et al. (2013) PLoS One 8:e64145. Poirier, S. et al. (2008) J. Biol. Chem. 283:2363. Levy, E. et al. (2013) Atherosclerosis 227:297. Le May, C. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:1484. Roubtsova, A. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:785. Chen, B. et. al. (2019) Curr.Top Med. Chem. 19:1790. Dixon, D.L. et. al. (2016) J. Clin. Lipidol. 10:1073.
Entrez Gene IDs	255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)
Alternate Names	EC 3.4.21, EC 3.4.21.111, FH3, FH3neural apoptosis regulated convertase 1, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC-1convertase subtilisin/kexin type 9 preproprotein, NARC1, NARC1EC 3.4.21.-, Neural apoptosis-regulated convertase 1, PC9, PCSK9, Proprotein Convertase 9, Subtilisin/kexin-like protease PC9, hypercholesterolemia, autosomal dominant 3, proprotein convertase subtilisin/kexin type 9

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Recombinant Cynomolgus Monkey PCSK9 His-tag Protein, CF

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