Recombinant Human LDLR Protein

Name: Recombinant Human LDLR Protein
Brand: R&D Systems
SKU: 2148-LD
Price: 405 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 2148-LD

Citations (9)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q59FQ1
Applications:	Binding Activity

View Full Technical Details for Recombinant Human LDLR Protein

Product Specifications for Recombinant Human LDLR Protein

Source	Mouse myeloma cell line, NS0-derived human LDL R protein Ala22-Arg788, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala22 & Asp193
Predicted Molecular Mass	85.6 kDa & 66.8 kDa
SDS-PAGE	125-140 kDa & 95-110 kDa, reducing conditions
Activity	Measured in a competitive binding assay. When human LDL is immobilized at 1 μg/mL (100 μL/well), Recombinant Human LDL R inhibits 50% binding of biotinylated recombinant human LDL R (0.5 μg/mL) at the concentration range of 0.4-2 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

2148-LD

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2148-LD/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: LDLR

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors (1-5). Members of the family are endocytic receptors, but can also co-regulate adjacent cell-surface signaling molecules (3, 4). Many proteins in the LDL R family are cleaved by extracellular proteases to release soluble forms to the circulation, and many of these soluble forms are active (1, 6). Mature LDL R is a 120-160 kDa (depending on glycosylation) type I transmembrane glycoprotein that contains cysteine-rich complement-like repeats (class A LDL domains), calcium-binding EGF repeats, and beta ‑propeller structures (class B LDL repeats) in the extracellular domain (ECD) (1-7). A membrane-proximal Ser/Thr-rich region shows extensive O-linked glycosylation (4, 8). A cytoplasmic NPxY motif links the LDL R to clathrin pits for endocytosis, and binds to select adaptor proteins (1, 4, 8). The human LDL R ECD shares 78%, 76%, 81% and 82% aa sequence identity with mouse, rat, bovine, and porcine LDL R, respectively. LDL R is constitutively and widely expressed. Its class A LDL domains near the N-terminus bind apoB and apoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively (1, 2, 4, 9). Hepatocyte LDL R is responsible for endocytosis and clearing of most plasma LDL cholesterol (2, 9). At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDL R to the cell surface (1, 4). Lack of LDL R expression or function causes familial hypercholesterolemia (FH) (4, 9, 10). The protease PCSK9 (proprotein convertase subtilisin/kexin type 9) can also cause increased plasma cholesterol by promoting LDL R degradation rather than recycling to the cell surface (10-12). Soluble forms of approximately 140 kDa and 28 kDa are reported to be released by phorbol esters or interferons, respectively (6, 7)

Item	Value
References	Go, G.W. and A. Mani (2012) Yale J. Biol. Med. 85:19. Ren, G. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1059. Bujo, H. and Y. Saito (2006) Arterioscler. Thromb. Vasc. Biol. 26:1246. Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461. Yamamoto, T. et al. (1984) Cell 39:27. Begg, M.J. et al. (2004) Eur. J. Biochem. 271:524. Fischer, D.G. et al. (1993) Science 262:250. Stolt, P.C. and H.H. Bock (2006) Cell. Signal. 18:1560. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5. De Castro-Oros, I. et al. (2010) Appl. Clin Genet. 3:53. Zhang, D.W. et al. (2008) Proc. Natl. Acad. Sci. USA 105:13045. Tavori, H. et al. (2013) Circulation 127:2403.
Long Name	Low Density Lipoprotein Receptor
Entrez Gene IDs	3949 (Human); 16835 (Mouse); 300438 (Rat); 396801 (Porcine); 102127361 (Cynomolgus Monkey)
Alternate Names	FH, FHC, LDL R, LDL receptor, LDLCQ2, LDLR, low density lipoprotein receptor, low-density lipoprotein receptor, low-density lipoprotein receptor class A domain-containing protein 3

Citations for Recombinant Human LDLR Protein (9)

Showing 1 - 9 of 9 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human LDLR Protein include:

Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Y Masuda et al. (2018), Generation and Characterization of a Novel Small Biologic Alternative to Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Antibodies, DS-9001a, Albumin Binding Domain-Fused Anticalin Protein J. Pharmacol. Exp. Ther., 365(2):368-378.
PMID: 29463608
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SA Felt et al. (2017), Ruxolitinib and polycation combination treatment overcomes multiple mechanisms of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus J. Virol., 0(0).
PMID: 28566376
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Kraehling J et al. (2016), Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells. Nat Commun, 7(0):13516.
PMID: 27869117
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Amirache F et al. (2014), Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor. Blood, 123(9):1422-4.
PMID: 24578496
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay

DeVay et al. (2013), Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2). J Biol Chem, 288(15):10805-18.
PMID: 23430252
Species: Human
Sample Types: Protein
Applications: Binding Assay

Kurasawa J et al. (2013), Mapping the binding region on the low density lipoprotein receptor for blood coagulation factor VIII. J Biol Chem, 288(30):22033-41.
PMID: 23754288
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Duff CJ et al. (2009), Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor. Biochem. J., 419(3):577-84.
PMID: 19196236
Species: Hamster
Sample Types: Whole Cells
Applications: Bioassay

Chien YJ et al. (2008), Bovine lactoferrin inhibits Japanese encephalitis virus by binding to heparan sulfate and receptor for low density lipoprotein. Virology, 379(1):143-51.
PMID: 18640695
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Fisher TS et al. (2007), Effects of pH and low density lipoprotein (LDL) on PCSK9-dependent LDL receptor regulation. J. Biol. Chem., 282(28):20502-12.
PMID: 17493938

Recombinant Human LDLR Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Human LDLR Protein

Formulation, Preparation and Storage

Carrier Free

2148-LD

Carrier

2148-LD/CF

Carrier Free

Reconstitution Calculator

Background: LDLR

Citations for Recombinant Human LDLR Protein (9)

Product Documents for Recombinant Human LDLR Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected