Intracellular NK Cell Activation Flow Cytometry Panel
R&D Systems, part of Bio-Techne | Catalog # FMC-P-005
Intracellular NK Cell Activation Flow Cytometry Panel
Key Product Details
Features
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Species
Human
Conjugate
Multiple
Scientific Data Images for Intracellular NK Cell Activation Flow Cytometry Panel
Intracellular NK Cell analysis over a 14-day time course.
NK cells were expanded from PBMCs using plate-bound Anti-Human NKp46 Monoclonal Antibody (MAB1850) in ExCellerate™ Human NK Cell Expansion Media, Xeno-Free (CCM032) plus Recombinant Human IL-2 (27 ng/mL, 202-IL), Recombinant Human IL-12 (10 ng/mL, 219-IL), Recombinant Human IL-18 (10 ng/mL, 9124-IL), Recombinant Human IL-21 (10 ng/mL, 8879-IL) for 13 days. NK cells were assessed on Days 0 and 13 for expression of human CD16 PerCP, Granzyme K Alexa Fluor® 594, Granzyme B Alexa Fluor 488, Perforin Alexa Fluor 750, CD56 Alexa Fluor 647, and CD3 Alexa Fluor 405. Cells were gated on singlets (FSC-A by FSC-H) and live cells and quadrants were set using isotype controls (not shown). Find Staining Protocol Here.Formulation, Preparation, and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Flow Cytometry Panel
Long Name
Flow Cytometry Panel
Additional Flow Cytometry Panel Products
Product Documents for Intracellular NK Cell Activation Flow Cytometry Panel
Product Specific Notices for Intracellular NK Cell Activation Flow Cytometry Panel
For research use only
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