ExCellerate™ Human NK Cell Expansion Media, Xeno-Free
Key Product Details
Features: | Media for the ex vivo expansion and culture of human Natural Killer lymphocytes under xeno-free conditions. |
Key Benefits: |
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Order Information
Shipping Information
$35 to United States
Products in stock will be shipped in 1-3 business days unless specified.
Assay Procedure
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FAQs
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Can serum-free media be used to expand NK cells in the Cloudz Human NK Cell Expansion Kit, Catalog # CLD004?
Yes. We have found a robust expansion of NK cells using Bio-Techne brand ExCellerate Human NK Cell Expansion Media, Xeno-Free, Catalog # CCM032. We have found that this medium is a superior alternative to other serum-free medias on the market.
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Can frozen PBMCs be used as the starting population for NK cell expansion with Catalog #s CLD004 and CCM032?
Frozen PBMCs work well as a starting population for subsequent NK cell expansion using CLD004. Cells should be thawed and washed twice at 300 x g for 5 minutes, to remove cryoprotectant. Gentle mixing is recommended. Allow the cells to rest for 1-4 hours in a 37 C incubator prior to expansion.
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What is the expected viability of cells after 9 days of culture, when following the Cloudz Human NK Cell Expansion Kit protocol with ExCellerate™ Human NK Cell Expansion Media, Xeno-Free media, catalog # CCM032?
The expected cell viability is 70-90%.