Can serum-free media be used to expand NK cells in the Cloudz Human NK Cell Expansion Kit, Catalog # CLD004?

Yes. We have found a robust expansion of NK cells using Bio-Techne brand ExCellerate Human NK Cell Expansion Media, Xeno-Free, Catalog # CCM032. We have found that this medium is a superior alternative to other serum-free medias on the market.

Can frozen PBMCs be used as the starting population for NK cell expansion with Catalog #s CLD004 and CCM032?

Frozen PBMCs work well as a starting population for subsequent NK cell expansion using CLD004. Cells should be thawed and washed twice at 300 x g for 5 minutes, to remove cryoprotectant. Gentle mixing is recommended. Allow the cells to rest for 1-4 hours in a 37 C incubator prior to expansion.

What is the expected viability of cells after 9 days of culture, when following the Cloudz Human NK Cell Expansion Kit protocol with ExCellerate™ Human NK Cell Expansion Media, Xeno-Free media, catalog # CCM032?

The expected cell viability is 70-90%.

ExCellerate™ Human NK Cell Expansion Media, Xeno-Free

Name: ExCellerate™ Human NK Cell Expansion Media, Xeno-Free
Brand: rnd
SKU: CCM032

Catalog # CCM032

Product Datasheet / COA / SDS

For robust NK cell expansion under xeno-free conditions.

Key Product Details

Features:	Media for the ex vivo expansion and culture of human Natural Killer lymphocytes under xeno-free conditions.
Key Benefits:	Robust NK cell expansion under serum-free and xeno-free conditions Optimized for use with the Cloudz™ NK Cell Activation Kit Compatible with a variety of NK cell activation methods Supports NK cell expansion from PBMCs or purified CD3⁺-depleted PBMCs

Order Information

Bio-Techne Corporation

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Shipping Information

$35 to United States

Products in stock will be shipped in 1-3 business days unless specified.

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Technical Data
Citations / Reviews / FAQs

Assay Procedure

ExCellerate™ Human NK Cell Expansion Media, Xeno-Free Summary

Media for the ex vivo expansion and culture of human Natural Killer lymphocytes under xeno-free conditions.

Why Use ExCellerate™ Human NK Cell Expansion Media?

Natural Killer (NK) cells play an important role in both the adaptive and innate immune responses that control infection, autoimmunity, and tumor immunosurveillance. Due to their intrinsic and non-specific anti-tumor activity, human NK cells have been employed as anti-cancer therapies. Robust expansion and maintenance of NK Cells is critical to facilitate NK cell therapy.

Versatility of ExCellerate Human NK Cell Expansion Media

ExCellerate Human NK Cell Expansion Media is optimized for NK cell expansion when used in combination with Cloudz Human NK Cell Expansion Kit and Recombinant Human Cytokines from R&D Systems. However, this versatile media also provides robust growth using a variety of cytokine combinations (IL-2, IL-12, IL-18, and IL-21) and cell activation methods, including particle, bead-based, or plate-bound Human NKp46/NCR1 Antibody. The activation and cytokine/growth factor combinations used with this media should be optimized by application or experimental protocol.

ExCellerate™ Human NK Cell Expansion Media, Xeno-Free Kit Contents

500 mL of ExCellerate Human NK Cell Expansion Media

Product Specifications

Species

Human

Limitations

FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
This reagent should not be used beyond the expiration date indicated on the label.
Results may vary due to variations among cells derived from different donors.

ExCellerate™ Human NK Cell Expansion Media, Xeno-Free Scientific Data Examples

	Fold Expansion of NK Cells Human PBMCs were expanded for 14 days with ExCellerate NK Expansion Media CCM032, Cloudz Human NK Cell Expansion Kit CLD004, and Bio-Techne cytokines (202-IL, 219-IL, 9124-IL, and 8879-IL). Cells were evaluated at Days 0, 7, 9, and 14 for fold expansion. Following 14 days of expansion, NK cells showed approximately 100-fold expansion.
	NK Cell Purity after 14-day expansion protocol. Human PBMCs were expanded for 14 days with ExCellerate NK Expansion Media CCM032, Cloudz Human NK Cell Expansion Kit CLD004, and Bio-Techne cytokines (202-IL, 219-IL, 9124-IL, and 8879-IL). Cells were evaluated at Days 0, 7, 9, and 14 for purity. Following 14 days of expansion, NK cells showed a purity of 94.4% of CD3^-CD56⁺ NK cells

Preparation & Storage

Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the recommended temperature.
Storage	Store the unopened product at -20 °C. Do not use past expiration date.

Citations for ExCellerate™ Human NK Cell Expansion Media, Xeno-Free

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FAQs

Can serum-free media be used to expand NK cells in the Cloudz Human NK Cell Expansion Kit, Catalog # CLD004?

Yes. We have found a robust expansion of NK cells using Bio-Techne brand ExCellerate Human NK Cell Expansion Media, Xeno-Free, Catalog # CCM032. We have found that this medium is a superior alternative to other serum-free medias on the market.
Can frozen PBMCs be used as the starting population for NK cell expansion with Catalog #s CLD004 and CCM032?

Frozen PBMCs work well as a starting population for subsequent NK cell expansion using CLD004. Cells should be thawed and washed twice at 300 x g for 5 minutes, to remove cryoprotectant. Gentle mixing is recommended. Allow the cells to rest for 1-4 hours in a 37 C incubator prior to expansion.
What is the expected viability of cells after 9 days of culture, when following the Cloudz Human NK Cell Expansion Kit protocol with ExCellerate™ Human NK Cell Expansion Media, Xeno-Free media, catalog # CCM032?

The expected cell viability is 70-90%.