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ExCellerate™ Human NK Cell Expansion Media, Xeno-Free Best Seller

Catalog # CCM032 | R&D Systems, Inc. a Bio-Techne Brand
20% Off ECM, Media, Buffers, and Serum with Promo Code CULTURE20! See T&C

For robust NK cell expansion under xeno-free conditions.

Key Product Details

Features: Media for the ex vivo expansion and culture of human Natural Killer lymphocytes under xeno-free conditions.
Key Benefits:
  • Robust NK cell expansion under serum-free and xeno-free conditions
  • Compatible with a variety of NK cell activation methods
  • Supports NK cell expansion from PBMCs or purified CD3+-depleted PBMCs
(4)
Human NK Cell expansion protocol over 2-week period.
Flow cytometry data of expanded human NK cells at 0, 8, 11, and 14 days.
Analysis of expansion fold and percent of total viable cells according to days in culture.
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Setting the standard in quality research reagents

Citations for ExCellerate™ Human NK Cell Expansion Media, Xeno-Free (1)

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    Name: Anonymous
    Verified Customer | Posted 02/02/2023

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FAQs for ExCellerate™ Human NK Cell Expansion Media, Xeno-Free

  • Can serum-free media be used to expand NK cells in the Cloudz Human NK Cell Expansion Kit, Catalog # CLD004? 

    Yes. We have found a robust expansion of NK cells using Bio-Techne brand ExCellerate Human NK Cell Expansion Media, Xeno-Free, Catalog # CCM032.  We have found that this medium is a superior alternative to other serum-free medias on the market. 

  • Can frozen PBMCs  be used as the starting population for NK cell expansion with Catalog #s CLD004 and CCM032?

    Frozen PBMCs work well as a starting population for subsequent NK cell expansion using CLD004. Cells should be thawed and washed twice at 300 x g for 5 minutes, to remove cryoprotectant. Gentle mixing is recommended. Allow the cells to rest for 1-4 hours in a 37 C incubator prior to expansion. 

  • What is the expected viability of cells after 9 days of culture, when following the Cloudz Human NK Cell Expansion Kit protocol with ExCellerate™ Human NK Cell Expansion Media, Xeno-Free media, catalog # CCM032?

    The expected cell viability is 70-90%. 

  • Can ExCellerate™ Human NK Cell Expansion Media, Xeno-Free, Catalog # CCM032, support the growth and expansion of NK T cells and INK T cells?

    We have only tested the expansion of NK cells using ExCellerate™ Human NK Cell Expansion Media, Xeno-Free. We have not tested for expansion of NK T cells or INK T cells.

Product Documents for ExCellerate™ Human NK Cell Expansion Media, Xeno-Free

Certificate of Analysis

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