Rat IL-18 DuoSet ELISA

Name: Rat IL-18 DuoSet ELISA
Brand: R&D Systems
SKU: DY521-05

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY521-05

Citations (3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Rat IL-18 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Rat IL-18 DuoSet ELISA

Rat IL-18 DuoSet ELISA, 5 Plate

Product Specifications for Rat IL-18 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Rat
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Rat IL-18 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant rat IL-18. The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-γ and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-γ that inhibits IgE production.

Item	Value
Long Name	Interleukin 18
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names	IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL-18, IL-18MGC12320, IL-1F4, IL-1g, IL18, IL1F4iboctadekin, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, interleukin 18 (interferon-gamma-inducing factor), interleukin-18

Citations for Rat IL-18 DuoSet ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Rat IL-18 DuoSet ELISA include:

Species: Rat
Sample Types: Cell Culture Supernates

C Liao et al. (2022), DUSP8/TAK1 signaling mediates neuropathic pain through regulating neuroinflammation and neuron death in a spinal nerve ligation (SNL) rat model International immunopharmacology, 113(0):109284.
PMID: 36279673
Species: Rat
Sample Types: Cell Culture Supernates

B Sun et al. (2021), Endogenous tRNA-derived small RNA (tRF3-Thr-AGT) inhibits ZBP1/NLRP3 pathway-mediated cell pyroptosis to attenuate acute pancreatitis (AP) Journal of Cellular and Molecular Medicine, 0(0).
PMID: 34643045
Species: Rat
Sample Types: Tissue Homogenates

J Zhou et al. (2020), MiR-199a modulates autophagy and inflammation in rats with cerebral infarction via regulating mTOR expression Eur Rev Med Pharmacol Sci, 24(11):6338-6345.
PMID: 32572931

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