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Human TNF-alpha DuoSet ELISA

Human TNF-alpha ELISA Standard Curve
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DY210-05
DY210

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Key Product Details

Assay Type: Solid Phase Sandwich ELISA
Assay Range: 15.6 - 1,000 pg/mL
Sample Types: Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human TNF-alpha DuoSet ELISA Features:
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

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Citations for Human TNF-alpha DuoSet ELISA (269)

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Customer Reviews (23)

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  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: THP-1 cell supernatant
    Verified Customer | Posted 03/24/2021
    24-hour stimulation of THP-1 cells to rotenone with or without TLR inhibitorR-squared value of 0.98 using sigmoidal 4-parameter logistic plot
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 03/24/2021
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: human macrophages
    Verified Customer | Posted 03/22/2021
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: human kidney tissue homogenate
    Verified Customer | Posted 12/20/2020
    TNFa was measured in the supernatant of human kidney tissue homogenate stimulated with LPS for 24 hours.
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: human monocyte-derived macrophages
    Verified Customer | Posted 12/20/2020
    This is a new lot of the Human TNF-alpha DuoSet ELISA kit. The results are reproducible and the quality of the kit consistent. Monocyte-derived human macrophages were treated with 20 µg/ml zymosan for 24 hours and TNFa was assessed in the supernatant.
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Monocyte-derived dendritic cells
    Verified Customer | Posted 11/28/2020
    TNFa secretion was measured in the supernatant of LPS-stimulated (100 ng/ml) cultured human dendritic cells after 18 hours.
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Monocytes
    Verified Customer | Posted 10/13/2020
    Monocytes were stimulated with LPS (100 ng/ml) for 24 hours. After 24 hours, TNFa was assessed in the supernatant.
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 08/11/2020
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: K562 human chronic myelogenous leukemia cell line
    Verified Customer | Posted 02/11/2020
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 01/15/2020
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Monocytes
    Verified Customer | Posted 11/04/2019
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Peripheral blood monocytes
    Verified Customer | Posted 08/08/2019
    TNFa levels in PBM supernatants after dIgG treatment.
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell Lysates
    Verified Customer | Posted 01/03/2019
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 01/03/2019
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 10/08/2018
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Purified Standard
    Verified Customer | Posted 09/11/2018
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 03/30/2018
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell Culture Media
    Verified Customer | Posted 01/25/2018
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 01/18/2018
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Verified Customer | Posted 12/18/2017
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: human sera
    Verified Customer | Posted 12/15/2017
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: U937 human histiocytic lymphoma cell line
    Verified Customer | Posted 10/17/2017
    Human TNF-alpha DuoSet ELISA Serum DY210
  • Human TNF-alpha DuoSet ELISA
    Name: Anonymous
    Sample Tested: human serum
    Verified Customer | Posted 06/13/2017
    Human TNF-alpha DuoSet ELISA Serum DY210

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