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Human CX3CL1/Fractalkine Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Assay Length: | 4.5 hours |
| Sample Type & Volume Required Per Well: | Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (50 uL), Urine (50 uL), Human Milk (100 uL) |
| Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk |
| Sensitivity: | 0.072 ng/mL |
| Assay Range: | 0.156 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk) |
| Human CX3CL1/Fractalkine Quantikine ELISA Kit Summary: | The Quantikine Human Fractalkine immunoassay is a 4.5 hour solid phase ELISA designed to measure Fractalkine in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Fractalkine and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Fractalkine showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fractalkine. |
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Linearity
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Human CX3CL1/Fractalkine ELISA Cell Culture Supernate/Saliva Standard Curve |
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Human CX3CL1/Fractalkine ELISA Serum/Plasma/Urine/Human Milk Standard Curve |
Citations for Human CX3CL1/Fractalkine Quantikine ELISA Kit (25)
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Citations are publications that use Bio-Techne products. Selected citations for Human CX3CL1/Fractalkine Quantikine ELISA Kit include:
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Species: Human
Sample Types: Nasal Lavage Fluid
S Ravichandr et al. (2021), Systemic and mucosal immune profiling in asymptomatic and symptomatic SARS-CoV-2-infected individuals reveal unlinked immune signatures Science Advances, 7(42):eabi6533.
PMID: 34644111 -
Species: Human
Sample Types: Urine
HR Jang et al. (2021), Early postoperative urinary MCP-1 as a potential biomarker predicting acute rejection in living donor kidney transplantation: a prospective cohort study Scientific Reports, 11(1):18832.
PMID: 34552150 -
Species: Human
Sample Types: Plasma
J Hesson et al. (2021), Cytomegalovirus Immunity, Inflammation and Cognitive Abilities in the Elderly Viruses, 13(11).
PMID: 34835127 -
Species: Human
Sample Types: Plasma
S Sendic et al. (2021), B cell and monocyte phenotyping: A quick asset to investigate the immune status in patients with IgA nephropathy PLoS ONE, 16(3):e0248056.
PMID: 33740017 -
Species: Human
Sample Types: Serum
A Helmke et al. (2020), Peritoneal dialysate-range hypertonic glucose promotes T cell IL-17 production that induces mesothelial inflammation Eur. J. Immunol., 0(0).
PMID: 32926407 -
Species: Human
Sample Types: Cell Culture Supernates
R Fu et al. (2020), Cathepsin S activation contributes to elevated CX3CL1 (fractalkine) levels in tears of a Sj�gren's syndrome murine model Sci Rep, 10(1):1455.
PMID: 31996771 -
Species: Human
Sample Types: Cell Culture Supernates
EJ Im et al. (2019), Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Nat Commun, 10(1):1387.
PMID: 30918259 -
Species: Human
Sample Types: Plasma
M Kashiwagi et al. (2019), Prognostic Value of Human Peripheral Monocyte Subsets for Future Coronary Events in Patients Without Significant Coronary Artery Stenosis Circ. J., 0(0).
PMID: 31511449 -
Species: Human
Sample Types: Plasma
A Mangold et al. (2019), Neutrophil extracellular traps and monocyte subsets at the culprit lesion site of myocardial infarction patients Sci Rep, 9(1):16304.
PMID: 31704966 -
Species: Human
Sample Types: Tissue Homogenates
AM Hoffmann-V et al. (2018), Augmented concentrations of CX3CL1 are associated with interstitial lung disease in systemic sclerosis PLoS ONE, 13(11):e0206545.
PMID: 30457999 -
Species: Rat
Sample Types: Serum
X Huang et al. (2017), Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-?B Mediators Inflamm., 2017(0):9734837.
PMID: 28634422 -
Species: Human
Sample Types: Plasma
M Kulkarni et al. (2016), Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection Open Forum Infect Dis, 3(4):ofw224.
PMID: 28066794 -
Species: Human
Sample Types: Plasma
(2016), Assessment of the Role of Metabolic Determinants on the Relationship between Insulin Sensitivity and Secretion PLoS ONE, 11(12):e0168352.
PMID: 28002466 -
Species: Human
Sample Types: Serum
Boag S et al. (2015), T lymphocytes and fractalkine contribute to myocardial ischemia/reperfusion injury in patients. J Clin Invest, 125(8):3063-76.
PMID: 26168217 -
Species: Human
Sample Types: Tissue Homogenates
Kassianos A et al. (2015), Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells. Kidney Int, 87(6):1153-63.
PMID: 25587706 -
Species: Human
Sample Types: Serum
(2012), Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis. BMC Infect Dis, 12(0):144.
PMID: 22731696 -
Species: Human
Sample Types: Serum
Ostroff R et al. (2012), Early detection of malignant pleural mesothelioma in asbestos-exposed individuals with a noninvasive proteomics-based surveillance tool. PLoS ONE, 7(10):e46091.
PMID: 23056237 -
Species: Human
Sample Types: Serum
Stolla M et al. (2012), Fractalkine is expressed in early and advanced atherosclerotic lesions and supports monocyte recruitment via CX3CR1. PLoS ONE, 7(8):e43572.
PMID: 22916279 -
Species: Human
Sample Types: Cell Culture Supernates
Gaudin F et al. (2012), Identification of the chemokine CX3CL1 as a new regulator of malignant cell proliferation in epithelial ovarian cancer. PLoS ONE, 6(7):e21546.
PMID: 21750716 -
Species: Human
Sample Types: CSF
Xu Y et al. (2012), Altered expression of CX3CL1 in patients with epilepsy and in a rat model. Am. J. Pathol., 180(5):1950-62.
PMID: 22464888 -
Species: Human
Sample Types: Urine
(2012), Activated innate immunity and the involvement of CX3CR1-fractalkine in promoting hematuria in patients with IgA nephropathy. Kidney Int., 82(5):548-60.
PMID: 22572859 -
Species: Human
Sample Types: Plasma
(2012), Fractalkine is an independent predictor of mortality in patients with advanced heart failure. Thromb Haemost, 108(6):1220-7.
PMID: 23014777 -
Species: Human
Sample Types: Plasma
Shah R et al. (2011), Fractalkine is a novel human adipochemokine associated with type 2 diabetes. Diabetes, 60(5):1512-8.
PMID: 21525510 -
Species: Human
Sample Types: Cell Culture Supernates
Matsumiya T et al. (2010), Characterization of synergistic induction of CX3CL1/fractalkine by TNF-alpha and IFN-gamma in vascular endothelial cells: an essential role for TNF-alpha in post-transcriptional regulation of CX3CL1. J. Immunol., 184(8):4205-14.
PMID: 20231691 -
Species: Human
Sample Types: Cell Culture Supernates
Huang YW et al. (2009), Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases. J. Biol. Chem., 284(43):29644-53.
PMID: 19723636
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Customer Reviews for Human CX3CL1/Fractalkine Quantikine ELISA Kit (4)
4.8 out of 5
4 customer ratings
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Sample Tested: SerumVerified Customer | Posted 11/07/2022
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Sample Tested: 7TD1 mouse hybridoma cell lineVerified Customer | Posted 07/12/2018
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Sample Tested: H69AR human small cell lung cancer cell lineVerified Customer | Posted 04/19/2018
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Sample Tested: Cell Culture SupernatesVerified Customer | Posted 10/14/2016
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Product Documents for Human CX3CL1/Fractalkine Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
