Skip to main content

StemXVivo Xeno-Free Human MSC Expansion Media

Catalog # CCM021 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Morphology of Mesenchymal Stem Cells (MSCs) Cultured in StemXVivo Xeno-Free Human MSC Expansion Media.
(2)
Expression of Mesenchymal Stem Cell (MSC) Markers in MSCs Expanded in R&D Systems StemXVivo® Xeno-Free Human MSC Expansion Media or a Competitor’s Xeno-Free MSC Expansion Media.
Loading...
Setting the standard in quality research reagents

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, mesenchymal stem cells can be analyzed for marker expression using the following procedure:

  • Resuspend cells in pre-warmed StemXVivo® Xeno-Free Human MSC Expansion Media
  • Plate the cells on Fibronectin-coated plates
  • Culture the cells at 37 °C and 5% CO2
 

 

Reagents Provided

Reagents supplied in the StemXVivo® Xeno-Free Human MSC Expansion Media (Catalog # CCM021)::

  • 500 mL of Xeno-Free Human MSC expansion media

 

Other Supplies Required

Reagents and Materials

  • Bone marrow-derived MSCs
  • Recombinant Human Fibronectin, ACFP (Catalog # ACFP4305)
  • Penicillin-Streptomycin (100X)
  • TrypLE™ Express (Invitrogen® or equivalent)
  • Phosphate-Buffered Saline (PBS)
  • 75 cm2 tissue culture flasks
  • 15 mL centrifuge tubes
  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 37 °C and 5% CO2 humidified incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 37 °C water bath

 

Protocol Overview

R&D Systems Protocol for Expanding and Subculturing MSCs in StemXVivo® Xeno-Free MSC Expansion Media (Catalog # CCM021)

Culturing Mesenchymal Stem Cells

Coat plates with Recombinant Human Fibronectin.

Coat plates with Recombinant Human Fibronectin.

Pre-warm the required amount of StemXVivo® Xeno-Free Human MSC Expansion Media to room temperature.

Pre-warm the required amount of media.

Resuspend 4.5-5.0 x 105 cells in 20 mL of the pre-warmed media.

Add the cell suspension to a Fibronectin-coated T75 flask.

Incubate the cells at 37 °C and 5% CO2 in a humidified atmosphere.

Replace spent media every 2-3 days with 20 mL of fresh, pre-warmed StemXVivo® Xeno-Free Human MSC Expansion Media.

Subculture the cells when they reach 80-90% confluency. Do not let the cultures become 100% confluent.

Resuspend cells in 20 mL of the pre-warmed media.
 

Subculturing Mesenchymal Stem Cells

Coat the plates with Recombinant Human Fibronectin.

Coat the plates with Recombinant Human Fibronectin.

Pre-warm StemXVivo® Xeno-Free Human MSC Expansion Media and TrypLE Express™ to room temperature.

Remove and discard the media from the flask(s).

Wash the cells once with PBS.

Detach the cells using TrypLE™ Express.

Pre-warm media.

Add 5 mL of StemXVivo® Xeno-Free Human MSC Expansion Media to the flask.

Transfer the cells to a 15 mL conical tube and centrifuge at 400 x g for 5 minutes.

Aspirate off the liquid.

Resuspend the cell pellet in a small amount of pre-warmed StemXVivo® Xeno-Free Human MSC Expansion Media.

Add 5 mL media to the flask.

Perform a cell count.

Perform a cell count.

Resuspend 4.5-5.0 x 105 cells in 20 mL of the pre-warmed StemXVivo® Xeno-Free Human MSC Expansion Media.

Add the cell suspension to a Fibronectin-coated T75 flask.

Incubate the cells at 37 °C and 5% CO2 in a humidified atmosphere.

Resuspend cells in pre-warmed media.

Citations for StemXVivo Xeno-Free Human MSC Expansion Media


Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

FAQs for StemXVivo Xeno-Free Human MSC Expansion Media

  • Is the appearance of crystals in StemXVivo® Xeno-free Human MSC Expansion Media (Catalog # CCM021) normal?

    Yes. The crystals are a calcium-containing precipitate and may form, to a limited degree, in this media. A limited amount of crystals will not impact MSC expansion. Avoiding multiple freeze-thaw cycles of the medium is helpful in reducing the amount of crystals.

  • Does StemXVivo Xeno-Free Human MSC Expansion Media, catalog # CCM021 contain purified Albumin?

    Yes, there is purified Human Serum Albumin (HSA) in the media. We have found it is essential for optimal performance and have not identified an alternative. We do not offer an albumin-free MSC media. CCM021 is Serum free and Xeno-free, but still contains human-derived components.

Product Documents for StemXVivo Xeno-Free Human MSC Expansion Media

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.