Resuspend 4.5-5.0 x 105 cells in 20 mL of the pre-warmed media.
Add the cell suspension to a Fibronectin-coated T75 flask.
Incubate the cells at 37 °C and 5% CO2 in a humidified atmosphere.
Replace spent media every 2-3 days with 20 mL of fresh, pre-warmed StemXVivo® Xeno-Free Human MSC Expansion Media.
Subculture the cells when they reach 80-90% confluency. Do not let the cultures become 100% confluent.