Human Endoglin/CD105 APC‑conjugated Antibody
Catalog # FAB10971A | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human Endoglin/CD105 APC‑conjugated Antibody
Immunogen
Glu26-Gly586
Accession # Q5T9B9
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Endoglin/CD105 APC‑conjugated Antibody
Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 APC-conjugated Mono-clonal Antibody (Catalog # FAB10971A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human Endoglin/CD105 APC‑conjugated Antibody
Flow Cytometry
Sample: U937 human histiocytic lymphoma cell line
Published Applications
Read 8 publications using FAB10971A in the following applications:
Formulation, Preparation and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Endoglin/CD105
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T betaRIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2‑5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1‑3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T betaRII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T betaRI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).
References
- Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
- ten Dijke, P. et al. (2008) Angiogenesis 11:79.
- Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
- Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
- Moody, J.L. et al. (2007) Stem Cells 25:2809.
- Velasco, S. et al. (2008) J. Cell Sci. 121:913.
- Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
- Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
- Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
- Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
- Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
- Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
- Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
- Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.
- Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Alternate Names
Gene Symbol
UniProt
Product Documents for Human Endoglin/CD105 APC‑conjugated Antibody
Product Specific Notices for Human Endoglin/CD105 APC‑conjugated Antibody
For research use only
Citations for Human Endoglin/CD105 APC‑conjugated Antibody (8)
Citations are publications that use Bio-Techne products. Selected citations for Human Endoglin/CD105 APC‑conjugated Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryS Di Franco et al. (2021-08-18), Adipose stem cell niche reprograms the colorectal cancer stem cell metastatic machinery Nature Communications, 2021-012(1):5006.
PMID: 34408135 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryH Watanabe et al. (2020-06-08), The neovascularization effect of dedifferentiated fat cells Sci Rep, 2020-010(1):9211.
PMID: 32514018 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryRI Dmitrieva et al. (2019-01-03), Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations Stem Cells Int, 2019-02019(0):5690345.
PMID: 30719048 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryTL Ramos et al. (2017-08-10), Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis PLoS ONE, 2017-012(8):e0182470.
PMID: 28796790 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryMuntion S et al. (2016-02-02), Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients. PLoS ONE, 2016-011(2):e0146722.
PMID: 26836120 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryL Ramos T et al. (2016-01-12), MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry. Cell Commun Signal, 2016-014(1):2.
PMID: 26754424 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKim S et al. (2013-12-20), Effects of long-term hypoxia and pro-survival cocktail in bone marrow-derived stromal cell survival. Stem Cells Dev, 2013-123(5):530-40.
PMID: 24147599 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryTran NT et al. (2011-09-27), Efficient differentiation of human pluripotent stem cells into mesenchymal stem cells by modulating intracellular signaling pathways in a feeder/serum-free system. Stem Cells Dev., 2011-021(7):1165-75.
PMID: 21793661
There are no citations that match your criteria.
FAQs for Human Endoglin/CD105 APC‑conjugated Antibody
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Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
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Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
A: We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.