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Human 5'‑Nucleotidase/CD73 PE‑conjugated Antibody
Catalog # FAB5795P | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 606112
Product Summary for Human 5'‑Nucleotidase/CD73 PE‑conjugated Antibody
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human 5'‑Nucleotidase/CD73
Trp27-Ile511
Accession # P21589
Trp27-Ile511
Accession # P21589
Specificity
Detects recombinant human 5'-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5'-Nucleotidase is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human 5'‑Nucleotidase/CD73 PE‑conjugated Antibody
Detection of 5'‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 PE-conjugated Monoclonal Antibody (Catalog # FAB5795P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Detection of 5'‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 PE-conjugated Monoclonal Antibody (Catalog # FAB5795P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (IC0041P). View our protocol for Staining Membrane-associated Proteins.Applications for Human 5'‑Nucleotidase/CD73 PE‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Sample: Human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 6 publications using FAB5795P in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: 5'-Nucleotidase/CD73
5'-Nucleotidase (also [ecto]-5'-nucleotidase/5'-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5'-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3+ Treg lymphocytes, FDCs and B cells. 5'-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5'-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5'‑Nucleotidase shares 88% aa identity with both mouse and rat 5'-Nucleotidase.
Product Specific Notices for Human 5'‑Nucleotidase/CD73 PE‑conjugated Antibody
For research use only
Citations for Human 5'‑Nucleotidase/CD73 PE‑conjugated Antibody (6)
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Citations are publications that use Bio-Techne products. Selected citations for Human 5'‑Nucleotidase/CD73 PE‑conjugated Antibody include:
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Species: Human
Sample Types: Whole Cells
A Cataldi et al. (2022-11-23), The Inhibition of the Inducible Nitric Oxide Synthase Enhances the DPSC Mineralization under LPS-Induced Inflammation International Journal of Molecular Sciences, 2022-123(23).
PMID: 36498888 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryA Borojevi? et al. (2022-02-18), Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling Biomolecules, 2022-012(2).
PMID: 35204824 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryM Gallorini et al. (2021-01-27), Liposomes embedded with differentiating factors as a new strategy for enhancing DPSC osteogenic commitment European cells & materials, 2021-041(0):108-120.
PMID: 33501637 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometrySJ Li et al. (2017-01-19), Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells Mol Med Rep, 2017-00(0).
PMID: 28112377 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryChristopher J Hewitt (2016-02-29), Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors Biotechnol J, 2016-011(4):473-86.
PMID: 26632496 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryBrady K et al. (2013-12-04), Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis. Stem Cells Dev, 2013-123(5):541-54.
PMID: 24172175
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FAQs for Human 5'‑Nucleotidase/CD73 PE‑conjugated Antibody
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Q: We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
A: We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
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