StemXVivo Serum-Free Dendritic Cell Base Media

Pre-optimized base media for successful dendritic cell culture.

Why Use Pre-optimized Reagents for the Production of Dendritic Cells from Monocytes and Subsequent Dendritic Cell Culture?

The efficiency of differentiation of common myeloid progenitors into dendritic cells can vary from one experiment to another depending on the media components, the supplements used to drive differentiation, and the quality of the starting cell population.

Inefficient differentiation represents a costly and time-consuming obstacle that can be minimized through the use of high quality media and differentiation supplements. StemXVivo^® Serum-Free Dendritic Cell Base Media is a defined media optimized for dendritic cell culture. The addition of Recombinant Human GM-CSF (Catalog # 215-GM) and Recombinant Human IL-4 (Catalog # 204-IL) generates a defined medium that efficiently drives differentiation of CD14⁺ monocytes into monocyte-derived dendritic cells (MoDCs).

StemXVivo^® Serum-Free Dendritic Cell Base Media:

• Optimized for dendritic cell culture.
• High lot-to-lot consistency decreases variation.
• Can be supplemented with user-defined growth factors and cytokines.
• Optimized for differentiation of CD14⁺ monocytes into dendritic cells.

Reagents supplied in the StemXVivo^® Serum-Free Dendritic Cell Base Media (Catalog # CCM003):

250 mL StemXVivo^® Serum-Free Dendritic Cell Base Media

Stability and Storage

Upon receipt, the Human StemXVivo^® Serum-Free Dendritic Cell Base Media should be stored at -20 °C in a manual defrost freezer. The media can be thawed at 2 °C to 8 °C or at room temperature. Thawed media can be aliquoted and stored at -20 °C in a manual defrost freezer for up to 3 months or used within a month when stored in the dark at 2 °C to 8 °C. Avoid repeated freeze-thaw cycles.

Precautions

The human origin-derived components used in this product have been derived from human plasma, which has been tested and found negative for antibodies to HIV-1/2, hepatitis B surface antigen (HBsAg), and hepatitis C virus (HCV). However, the medium should be handled as if potentially infectious. Safe laboratory procedures should be followed and protective clothing should be worn when handling this media. The acute and chronic effects of over-exposure to this media are unknown.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• This reagent should not be used beyond the expiration date indicated on the label.
• Results may vary due to variations between dendritic cell precursors/progenitor cells derived from different donors.

HSC Differentiation & Lineage Marker Schematic View Interactive Pathway

The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long mainte­nance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continu­ously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage deple­tion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.