Human B7-2/CD86 Fluorescein-conjugated Antibody

Name: Human B7-2/CD86 Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB141F

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB141F

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Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑2/CD86
Applications:	Flow Cytometry
Label:	Fluorescein (Excitation=488 nm, Emission=515-545 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 37301
Specificity:	Detects human B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.

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Applications for Human B7-2/CD86 Fluorescein-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human B7-2/CD86 Fluorescein-conjugated Antibody

	Flow Cytometry Detection of B7‑2/CD86 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F) or (B) Mouse IgG₁Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: B7-2/CD86

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Human B7-2 is a 329 amino acid (aa) protein containing a putative 23 aa signal peptide, a 224 aa extracellular domain, a 21 aa transmembrane domain, and a 61 aa cytoplasmic domain. Human B7-2 and B7-1 share 26% amino acid identity. Human and mouse B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

Item	Value
References	Azuma, M. et al. (1993) Nature 366:76. Freeman, G.J. et al. (1993) Science 262:909. Freeman, G. et al. (1991) J. Exp. Med. 174:625. Selvakumar, A. et al. (1993) Immunogenetics 38:292. Chen, C. et al. (1994) J. Immunol. 152:4929. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs	942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)
Alternate Names	Activation B7-2 antigen, B-lymphocyte activation antigen B7-2, B7-2, B7-2 antigen, B70, B72, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86, CD86 antigen, CD86 molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86

Citations for Human B7-2/CD86 Fluorescein-conjugated Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human B7-2/CD86 Fluorescein-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kraetzel K et al. (2008), NKG2D-dependent effector function of bronchial epithelium-activated alloreactive T-cells. Eur. Respir. J., 32(3):563-70.
PMID: 18417514

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Human B7-2/CD86 Fluorescein-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human B7-2/CD86 Fluorescein-conjugated Antibody

Scientific Data Examples for Human B7-2/CD86 Fluorescein-conjugated Antibody

Flow Cytometry