N-2 MAX Media Supplement (100X)

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

N-2 MAX Media Supplement (100X)_AR009
(5)
Neural Progenitor Cells Expanded with N-2 Plus Media Supplement Express Nestin and SOX2.
Lot Consistency of N-2 MAX Media Supplement
Verification of Neural Progenitor Cell Multipotency Following Expansion with N-2 MAX Media Supplement. 
N-2 MAX Comparable to GMP N-2 MAX_AR009_AR016
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AR009
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Setting the standard in quality research reagents
  • Preparation & Storage

    • Preparation & Storage

    Shipping Conditions The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
    Storage Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

    Assay Procedure

    Refer to the product datasheet for complete product details.

     

    Briefly, completed N-2 MAX-supplemented neural cell medium is prepared using the following procedure:

    • Dilute the media supplement in basal media
    • Store completed media
    • Use within 2 weeks
     

     

    Reagents Provided

    Reagents provided in the N-2 MAX Media Supplement (Catalog # AR009):

    • Recombinant Human Insulin (2,500 µg/mL)
    • Human Transferrin (10,000 µg/mL)
    • Putrescine (1,611 µg/mL)
    • Selenite (0.52 µg/mL)
    • Progesterone (0.63 µg/mL)

     

    Other Supplies Required

    Reagents

    • DMEM/F-12 (Invitrogen®, Catalog # 12500-062) or a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029)
    • Glucose
    • Glutamine
    • NaHCO3
    • Penicillin-Streptomycin (100X)
    • Deionized or distilled water

    Materials

    • Serological pipettes
    • Pipettes and pipette tips
    • 2 µm filter unit

    Equipment

    • 2 °C to 8 °C refrigerator
     

     

    Protocol Overview

    Option 1: Mix the following components with deionized or distilled water to make 500 mL of medium.

    Component Amount (mg/mL)
    DMEM/F-12 6 g
    Glucose 0.775 g
    Glutamine 0.0365 g
    NaHCO3 0.854 g
    N-2 MAX Media Supplement 5 mL

     

    Adjust the pH to 7.2. Filter the solution (2 µm filter unit), and add 5 mL of 100X sterile Penicillin-Streptomycin solution. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

    Option 2: Dilute 100-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

    Invitrogen is a registered trademark of Invitrogen Corp.

  • Citations
  • Reviews
  • FAQs

    • Citations for N-2 MAX Media Supplement (100X) (23)

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    Customer Reviews for N-2 MAX Media Supplement (100X) (12)

    4.8 out of 5
    12 customer ratings
    5 stars
    75%
    4 stars
    25%
    3 stars
    0%
    2 stars
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    • N-2 MAX Media Supplement (100X)
      Name: Anonymous
      Verified Customer | Posted 03/04/2021
      I used this product to grow iPSC for differentiation. This product works excellent.
      N-2 MAX Media Supplement (100X) AR009
    • N-2 MAX Media Supplement (100X)
      Name: Anonymous
      Verified Customer | Posted 02/17/2021
      I use this product to grow stem cells. This product is excellent.
      N-2 MAX Media Supplement (100X) AR009
    • N-2 MAX Media Supplement (100X)
      Name: Anonymous
      Verified Customer | Posted 12/28/2020
      Cultured cells with N2 supplement and they grew well..
      N-2 MAX Media Supplement (100X) AR009
    • N-2 MAX Media Supplement (100X)
      Name: Anonymous
      Verified Customer | Posted 01/30/2020
      Differentiated human iPS cells into NPCs
      N-2 MAX Media Supplement (100X) AR009
    • N-2 MAX Media Supplement (100X)
      Name: Anonymous
      Verified Customer | Posted 08/15/2019
      We cultured with N2 supplement.It's good for cell growth.
      N-2 MAX Media Supplement (100X) AR009
    • N-2 MAX Media Supplement (100X)
      Name: Punitha Senthil
      Verified Customer | Posted 07/07/2018
      N-2 MAX Media Supplement (100X) AR009
    • N-2 MAX Media Supplement (100X)
      Name: Dharmaraja Allimuthu
      Verified Customer | Posted 11/01/2017
      IPSCs cultured on a polymer supported plate for differentiation
      N-2 MAX Media Supplement (100X) AR009
    • N-2 MAX Media Supplement (100X)
      Name: Supriya Mahajan
      Verified Customer | Posted 05/30/2017
      I have previously used N2 Neuroplex supplement from Gemimi Bio (Cat # 400-163) to culture neurons and this sample from R&D Systems work equally well. We will be switching to N-2 MAX.
      N-2 MAX Media Supplement (100X) AR009
    • N-2 MAX Media Supplement (100X)
      Name: Anonymous
      Verified Customer | Posted 12/06/2016
      N2 Max is a component of our base media for differentiating mouse and human stem cells to neural progenitor cells for modeling neural development and disease.
    • N-2 MAX Media Supplement (100X)
      Name: Anonymous
      Verified Customer | Posted 12/06/2016
      I use this product to grow Neural Progenitor Cells (NPC). This product is excellent. The cells grow very well in this media.
    • N-2 MAX Media Supplement (100X)
      Name: Scott Schachtele
      Verified Customer | Posted 12/06/2016
      We use in our media for neural stem cell expansion.
    • N-2 MAX Media Supplement (100X)
      Name: Scott Schachtele
      Verified Customer | Posted 12/06/2016
      N2 max supplement was used throughout the whole process of generation, culture, and maintenance of human and mouse iPS-derived dopaminergic neurons. The product performed well and better than one produced by another company.

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    FAQs for N-2 MAX Media Supplement (100X)

    • What is the difference between N-2 Plus Media Supplement (Catalog # AR003) and N-2 MAX Media Supplement (Catalog # AR009)?

      The N-2 Plus Media Supplement (Catalog # AR003) contains Bovine Insulin whereas N-2 MAX Media Supplement (Catalog # AR009) contains Recombinant Human Insulin. All other media components and concentrations in N-2 Plus and N-2 MAX are the same. These two products perform comparably in side-by-side testing. Due to a limited supply of bovine insulin, the products are priced differently.

    • Does N-2 MAX Media Supplement (Catalog # AR009) contain any components that are calcium salts?

      No.

    • Does N-2 MAX Media Supplement (Catalog # AR009) contain any proteins that are produced using Baculovirus?

      No.

    • an the N-2 MAX Media Supplement be stored frozen as 1 mL aliquots?

      Yes. The N-2 MAX Media Supplement may be stored frozen as 1 mL aliquots.  The frozen aliquots should not undergo multiple freeze-thaws before use.

    • Does the N-2 MAX Media Supplement contain any animal-derived components other than Human Transferrin?

      No. The N-2 MAX Media Supplement does not contain any animal-derived components other than Human Transferrin. GMP N-2 MAX Media Supplement is an animal-free supplement (Catalog # AR016).

    • How many freeze/thaw cycles can N-2 MAX Media Supplement (100X), Catalog # AR009, undergo? 

      The N-2 MAX Media Supplement should be able to undergo one freeze/thaw cycyle as long as the product is used witin the expiration date. 

    Product Documents for N-2 MAX Media Supplement (100X)

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.