N-2 MAX Media Supplement (100X)
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, completed N-2 MAX-supplemented neural cell medium is prepared using the following procedure:
- Dilute the media supplement in basal media
- Store completed media
- Use within 2 weeks
Reagents provided in the N-2 MAX Media Supplement (Catalog # AR009):
- Recombinant Human Insulin (2,500 µg/mL)
- Human Transferrin (10,000 µg/mL)
- Putrescine (1,611 µg/mL)
- Selenite (0.52 µg/mL)
- Progesterone (0.63 µg/mL)
Reagents
- DMEM/F-12 (Invitrogen®, Catalog # 12500-062) or a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029)
- Glucose
- Glutamine
- NaHCO3
- Penicillin-Streptomycin (100X)
- Deionized or distilled water
Materials
- Serological pipettes
- Pipettes and pipette tips
- 2 µm filter unit
Equipment
- 2 °C to 8 °C refrigerator
Option 1: Mix the following components with deionized or distilled water to make 500 mL of medium.
Component | Amount (mg/mL) |
DMEM/F-12 | 6 g |
Glucose | 0.775 g |
Glutamine | 0.0365 g |
NaHCO3 | 0.854 g |
N-2 MAX Media Supplement | 5 mL |
Adjust the pH to 7.2. Filter the solution (2 µm filter unit), and add 5 mL of 100X sterile Penicillin-Streptomycin solution. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.
Option 2: Dilute 100-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.
Invitrogen is a registered trademark of Invitrogen Corp.
Citations for N-2 MAX Media Supplement (100X) (23)
Citations are publications that use Bio-Techne products. Selected citations for N-2 MAX Media Supplement (100X) include:
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Species: Human
Sample Types: Whole Cells
Applications: Cell CultureM Maruyama et al. (2023), Establishment of a novel in vitro co-culture system of enteric neurons and Caco-2 cells for evaluating the effect of enteric nervous system on transepithelial transport of drugs International journal of pharmaceutics, 633(0):122617.
PMID: 36657552 -
Species: Rat
Sample Types: Whole Cells
Applications: BioassayI Horinokita et al. (2022), Effect of Progranulin on Proliferation and Differentiation of Neural Stem/Progenitor Cells after Oxygen/Glucose Deprivation International Journal of Molecular Sciences, 23(4).
PMID: 35216064 -
Species: Human
Sample Types: Organoids
Applications: BioassayW Shin et al. (2022), 3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert Nature Protocols, 0(0).
PMID: 35110737 -
Species: Mouse
Sample Types: Whole Cells
S Martin et al. (2022), Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay Nature Communications, 13(1):5003.
PMID: 36008413 -
Species: Deer
Sample Types: Tissue Homogenates
Applications: RT-QuICKR Burgener et al. (2022), Diagnostic testing of chronic wasting disease in white-tailed deer (Odocoileus virginianus) by RT-QuIC using multiple tissues PLoS ONE, 17(11):e0274531.
PMID: 36383520 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureY Hori et al. (2021), Aromatic-Turmerone Analogs Protect Dopaminergic Neurons in Midbrain Slice Cultures through Their Neuroprotective Activities Cells, 10(5).
PMID: 34063571 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayM Lattke et al. (2021), Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture Nature Communications, 12(1):4335.
PMID: 34267208 -
Species: Mouse
Sample Types: Organoid
MW Dougherty et al. (2020), Gut microbiota maturation during early human life induces enterocyte proliferation via microbial metabolites BMC Microbiol., 20(1):205.
PMID: 32652929 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayKC Allan et al. (2020), Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function Cell Stem Cell, 0(0).
PMID: 33091368 -
Species: Mouse
Sample Types: Whole Tissue
Applications: Organ CultureG Casey et al. (2020), Self-complementarity in adeno-associated virus enhances transduction and gene expression in mouse cochlear tissues PLoS ONE, 15(11):e0242599.
PMID: 33227033 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayA Tripathi et al. (2019), Oligodendrocyte Intrinsic miR-27a Controls Myelination and Remyelination Cell Rep, 29(4):904-919.e9.
PMID: 31644912 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Song et al. (2019), Controlling properties of human neural progenitor cells using 2D and 3D conductive polymer scaffolds Sci Rep, 9(1):19565.
PMID: 31863072 -
Z Hubler et al. (2018), Accumulation of 8,9-unsaturated sterols drives oligodendrocyte formation and remyelination Nature, 0(0).
PMID: 30046109 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayF Chen et al. (2018), Neutrophils Promote Amphiregulin Production in Intestinal Epithelial Cells through TGF-? and Contribute to Intestinal Homeostasis J. Immunol., 0(0).
PMID: 30171165 -
Species: Mouse
Sample Types: Whole Cells
C Stork et al. (2018), Developmental Xist induction is mediated by enhanced splicing Nucleic Acids Res., 0(0).
PMID: 30496473 -
Species: Mouse
Sample Types: Whole Cells
J Liu et al. (2017), Isolation and characterization of string-forming female germline stem cells from ovaries of neonatal mice J. Biol. Chem., 0(0).
PMID: 28827310 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayZS Nevin et al. (2017), Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes Am. J. Hum. Genet., 100(4):617-634.
PMID: 28366443 -
Species: Human
Sample Types: Whole Cells
Margriet A Huisman (2016), Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery Cytotechnology, 68(5):1849-58.
PMID: 26702932 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayNajm et al. (2013), Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol, 31(5):426-33.
PMID: 23584611 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayWoods D et al. (2013), Isolation, characterization and propagation of mitotically active germ cells from adult mouse and human ovaries. Nat Protoc, 8(5):966-88.
PMID: 23598447 -
Species: Human
Sample Types: Whole Cells
Linley AJ et al. (2012), The helicase HAGE expressed by malignant melanoma-initiating cells is required for tumor cell proliferation in vivo. J. Biol. Chem., 287(17):13633-43.
PMID: 22393060 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayNevin Z et al. (0), Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes. Am J Hum Gene100(4):617-634.
PMID: 28366443 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassaySchomann T et al. (0), Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants. PLoS ON12(10):e0187183.
PMID: 29084289
There are no citations that match your criteria.
Customer Reviews for N-2 MAX Media Supplement (100X) (12)
Customer Images
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Verified Customer | Posted 03/04/2021I used this product to grow iPSC for differentiation. This product works excellent.
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Verified Customer | Posted 02/17/2021I use this product to grow stem cells. This product is excellent.
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Verified Customer | Posted 12/28/2020Cultured cells with N2 supplement and they grew well..
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Verified Customer | Posted 01/30/2020Differentiated human iPS cells into NPCs
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Verified Customer | Posted 08/15/2019We cultured with N2 supplement.It's good for cell growth.
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Verified Customer | Posted 07/07/2018
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Verified Customer | Posted 11/01/2017IPSCs cultured on a polymer supported plate for differentiation
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Verified Customer | Posted 05/30/2017I have previously used N2 Neuroplex supplement from Gemimi Bio (Cat # 400-163) to culture neurons and this sample from R&D Systems work equally well. We will be switching to N-2 MAX.
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Verified Customer | Posted 12/06/2016N2 Max is a component of our base media for differentiating mouse and human stem cells to neural progenitor cells for modeling neural development and disease.
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Verified Customer | Posted 12/06/2016I use this product to grow Neural Progenitor Cells (NPC). This product is excellent. The cells grow very well in this media.
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Verified Customer | Posted 12/06/2016We use in our media for neural stem cell expansion.
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Verified Customer | Posted 12/06/2016N2 max supplement was used throughout the whole process of generation, culture, and maintenance of human and mouse iPS-derived dopaminergic neurons. The product performed well and better than one produced by another company.
There are no reviews that match your criteria.
FAQs for N-2 MAX Media Supplement (100X)
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What is the difference between N-2 Plus Media Supplement (Catalog # AR003) and N-2 MAX Media Supplement (Catalog # AR009)?
The N-2 Plus Media Supplement (Catalog # AR003) contains Bovine Insulin whereas N-2 MAX Media Supplement (Catalog # AR009) contains Recombinant Human Insulin. All other media components and concentrations in N-2 Plus and N-2 MAX are the same. These two products perform comparably in side-by-side testing. Due to a limited supply of bovine insulin, the products are priced differently.
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Does N-2 MAX Media Supplement (Catalog # AR009) contain any components that are calcium salts?
No.
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Does N-2 MAX Media Supplement (Catalog # AR009) contain any proteins that are produced using Baculovirus?
No.
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an the N-2 MAX Media Supplement be stored frozen as 1 mL aliquots?
Yes. The N-2 MAX Media Supplement may be stored frozen as 1 mL aliquots. The frozen aliquots should not undergo multiple freeze-thaws before use.
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Does the N-2 MAX Media Supplement contain any animal-derived components other than Human Transferrin?
No. The N-2 MAX Media Supplement does not contain any animal-derived components other than Human Transferrin. GMP N-2 MAX Media Supplement is an animal-free supplement (Catalog # AR016).
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How many freeze/thaw cycles can N-2 MAX Media Supplement (100X), Catalog # AR009, undergo?
The N-2 MAX Media Supplement should be able to undergo one freeze/thaw cycyle as long as the product is used witin the expiration date.