TGF-beta 3 Antibody

Name: TGF-beta 3 Antibody
Brand: R&D Systems
SKU: AB-244-NA

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AB-244-NA

Citations (4)

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Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant chicken TGF‑ beta 3 and recombinant human TGF‑ beta 3 Ala301-Ser412 (Tyr340Phe) Accession # P10600
Applications:	Block/Neutralize
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects TGF‑ beta 3 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant amphibian TGF-beta 5 is observed and less than 5% cross-reactivity with recombinant human (rh) TGF-beta 1, rhTGF-beta 2 and rhLAP (TGF-beta 1) is observed.

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Applications for TGF-beta 3 Antibody

Item	Value
Neutralization	Measured by its ability to neutralize TGF‑ beta 3 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 0.1 ng/mL Recombinant Human TGF‑ beta 3 and 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for TGF-beta 3 Antibody

	Neutralization TGF‑ beta 3 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 3 Antibody. Recombinant Human TGF-beta 3 (Catalog # 243-B3) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-TGF-beta 3 Polyclonal Antibody (Catalog # AB-244-NA). The ND₅₀ is typically 1-3 µg/mL.
	Western Blot Detection of Human TGF‑ beta 3 by Western Blot. Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-TGF-beta 3 Polyclonal Antibody (Catalog # AB-244-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF-beta 3 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human TGF‑ beta 3 by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF-beta 3 at approximately 64 kDa (as indicated) using 50 µg/mL of Goat Anti-TGF-beta 3 Polyclonal Antibody (Catalog # AB-244-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 1 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: TGF-beta 3

TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8‑10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity (1). TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

Item	Value
References	Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982. Wahl, S.M. (2006) Immunol. Rev. 213:213. Chang, H. et al. (2002) Endocr. Rev. 23:787. Lin, J.S. et al. (2006) Reproduction 132:179. Hinck, A.P. et al. (1996) Biochemistry 35:8517. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261. Derynck, R. et al. (1988) EMBO J. 7:3737. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name	Transforming Growth Factor beta 3
Entrez Gene IDs	7043 (Human); 21809 (Mouse); 25717 (Rat)
Alternate Names	ARVD, ARVD1, FLJ16571, LDS5, RNHF, TGF-beta 3, TGF-beta-3, TGF-beta3, TGFB3, TGFbeta 3, transforming growth factor beta-3, transforming growth factor, beta 3

Citations for TGF-beta 3 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for TGF-beta 3 Antibody include:

Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

YH Wu et al. (2021), COL11A1 activates cancer-associated fibroblasts by modulating TGF-&beta3 through the NF-&kappaB/IGFBP2 axis in ovarian cancer cells Oncogene, 0(0).
PMID: 34117361
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Brown GD et al. (2010), Matrix metalloproteinase-25 has a functional role in mouse secondary palate development and is a downstream target of TGF-beta3. BMC Dev. Biol., 10(0):93.
PMID: 20809987
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Shintani Y et al. (2007), Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling. Am. J. Respir. Cell Mol. Biol., 38(1):95-104.
PMID: 17673689
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot

Mozes MM et al. (1999), Isoform specificity of commercially-available anti-TGF-beta antibodies. J. Immunol. Methods, 225(1):87-93.
PMID: 10365785

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TGF-beta 3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for TGF-beta 3 Antibody

Scientific Data Examples for TGF-beta 3 Antibody

Neutralization

Western Blot

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: TGF-beta 3

Citations for TGF-beta 3 Antibody (4)

Product Documents for TGF-beta 3 Antibody

Product Datasheets

COA

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SDS

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