TGF-beta 3 Antibody

Name: TGF-beta 3 Antibody
Brand: R&D Systems
SKU: MAB243
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB243

Citations (6)

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Immunogen:	Spodoptera frugiperda, Sf 21 (baculovirus) derived recombinant human TGF-beta 3 Ala301-Ser412 (Tyr340Phe) Accession # P10600
Applications:	Block/Neutralize, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 20724
Specificity:	Detects TGF-beta 3 from multiple species in direct ELISAs and Western blots. In Western blots, less than 25% cross-reactivity with recombinant human (rh) TGF‑ beta 1.2 and rhTGF-beta 2 is observed, and less than 2% cross‑reactivity with recombinant amphibian TGF-beta 5 and recombinant human TGF-beta 1 is observed. Neutralizes the biological activity of TGF-beta 3 but not TGF-beta 1, TGF-beta 2, or TGF-beta 5.

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Applications for TGF-beta 3 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human TGF-beta 3 (Catalog # 243-B3) under non-reducing conditions only
Immunohistochemistry	8-25 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize TGF‑ beta 3 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 0.1 ng/mL Recombinant Human TGF‑ beta 3 and 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for TGF-beta 3 Antibody

	Neutralization TGF‑ beta 3 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 3 Antibody. Recombinant Human TGF-beta 3 (Catalog # 243-B3) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-TGF-beta 3 Monoclonal Antibody (Catalog # MAB243). The ND₅₀ is typically 0.1-0.3 µg/mL.
	Immunohistochemistry TGF‑ beta 3 in Human Breast Cancer Tissue. TGF-beta 3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-TGF-beta 3 Monoclonal Antibody (Catalog # MAB243) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: TGF-beta 3

TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF‑ beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8‑10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity.(1) TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

Item	Value
References	Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982. Wahl, S.M. (2006) Immunol. Rev. 213:213. Chang, H. et al. (2002) Endocr. Rev. 23:787. Lin, J.S. et al. (2006) Reproduction 132:179. Hinck, A.P. et al. (1996) Biochemistry 35:8517. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261. Derynck, R. et al. (1988) EMBO J. 7:3737. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name	Transforming Growth Factor beta 3
Entrez Gene IDs	7043 (Human); 21809 (Mouse); 25717 (Rat)
Alternate Names	ARVD, ARVD1, FLJ16571, LDS5, RNHF, TGF-beta 3, TGF-beta-3, TGF-beta3, TGFB3, TGFbeta 3, transforming growth factor beta-3, transforming growth factor, beta 3

Citations for TGF-beta 3 Antibody (6)

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Citations are publications that use Bio-Techne products. Selected citations for TGF-beta 3 Antibody include:

Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Okamura T et al. (2015), TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses. Nat Commun, 6(0):6329.
PMID: 25695838
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Yin H et al. (2013), Expression profiling of nuclear receptors identifies key roles of NR4A subfamily in uterine fibroids. Mol Endocrinol, 27(5):726-40.
PMID: 23550059
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Ozturk F et al. (2013), Systematic analysis of palatal transcriptome to identify cleft palate genes within TGFbeta3-knockout mice alleles: RNA-Seq analysis of TGFbeta3 Mice. BMC Genomics, 14(0):113.
PMID: 23421592
Species: Canine
Sample Types: Whole Cells
Applications: Neutralization

Gregory PA et al. (2011), An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Mol. Biol. Cell, 22(10):1686-98.
PMID: 21411626
Species: Chicken
Sample Types: Whole Tissue
Applications: IHC

Arciniegas E et al. (2004), Differential versican isoforms and aggrecan expression in the chicken embryo aorta. Anat Rec A Discov Mol Cell Evol Biol, 279(1):592-600.
PMID: 15224401
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Liu X et al. (2002), Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am. J. Physiol. Lung Cell Mol. Physiol., 282(5):L1049-56.
PMID: 11943670

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TGF-beta 3 Antibody

Name: Anonymous

Sample Tested: Recombinant protein

Species: Human

Verified Customer | Posted 03/28/2019

direct ELISA to check specificity using in-house produced recombinant proteins

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TGF-beta 3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for TGF-beta 3 Antibody

Scientific Data Examples for TGF-beta 3 Antibody

Neutralization

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: TGF-beta 3

Citations for TGF-beta 3 Antibody (6)

Customer Reviews for TGF-beta 3 Antibody (1)

Customer Images

TGF-beta 3 Antibody

Product Documents for TGF-beta 3 Antibody

Product Datasheets

COA

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