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TGF-beta 2 Antibody

Bio-Techne includes R&D Systems | Catalog # AB-112-NA

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AB-112-NA

Key Product Details

Species Reactivity

Multi-Species

Applications

Neutralization, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for TGF-beta 2 Antibody

Immunogen

Porcine platelet-derived TGF-beta 2

Specificity

Detects TGF-beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody is highly specific for TGF-beta 2 andTGF‑ beta1.2 but will cross-react with other TGF-beta variants with at least 100-fold lower sensitivity. It will also neutralize the biological activity of TGF-beta 1.2, at a 50 fold higher IgG concentration.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TGF-beta 2 Antibody

Detection of Human TGF-beta 2 antibody by Western Blot.

Detection of Human TGF‑ beta2 by Western Blot.

Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-112-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF-beta 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human TGF-beta 2 antibody by Simple WesternTM.

Detection of Human TGF‑ beta2 by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF-beta 2 at approximately 63 kDa (as indicated) using 2.5 µg/mL of Goat Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-112-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
TGF‑ beta2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta2 Antibody.

TGF‑ beta2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta2 Antibody.

Porcine TGF-beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-112-NA). The ND50 is typically 0.3-1.5 µg/mL.

Applications for TGF-beta 2 Antibody

Application
Recommended Usage

Simple Western

2.5 µg/mL
Sample: Human heart tissue and human breast cancer tissue

Western Blot

0.25 µg/mL
Sample:   Human heart tissue and human breast cancer tissue

Neutralization

Measured by its ability to neutralize TGF‑ beta2 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 1 ng/mL Porcine TGF‑ beta2 and 7.5 ng/mL Recombinant Mouse IL‑4.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 6 publications using AB-112-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 2

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

References

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. deMartin, R. et al. (1987) EMBO J. 6:3673.1.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Long Name

Transforming Growth Factor beta 2

Alternate Names

TGFB2, TGFbeta 2

Entrez Gene IDs

7042 (Human); 21808 (Mouse); 397084 (Porcine)

Gene Symbol

TGFB2

Additional TGF-beta 2 Products

Product Documents for TGF-beta 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for TGF-beta 2 Antibody

For research use only

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