Detects rat IL-1 beta/IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.8% cross-reactivity with recombinant mouse (rm) IL‑1 beta is observed and less than 0.2% cross-reactivity with recombinant human (rh) IL-1 alpha, recombinant rat IL-1 alpha, recombinant porcine (rp) IL‑1 alpha, rhIL‑1 beta, rpIL‑1 beta, rhIL-1 RA, rmIL-1 RA, rpIL-1 RA, rhIL-1 RII, rmIL-1 RI Fc Chimera, rhIL-1 Rrp2 Fc Chimera, and rhIL-1 RAcP Fc Chimera is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat IL-1 beta/IL-1F2 Biotinylated Antibody

IL‑1 beta/IL‑1F2 in Rat Splenocytes.

IL-1 beta/IL-1F2 was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Goat Anti-Rat IL-1 beta/IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF501) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Rat IL-1 beta / IL-1F2 ELISA Standard Curve

Recombinant Rat IL-1 beta/IL-1F2 (Catalog # 501-RL) was serially diluted and captured by Goat Anti-Rat IL-1 beta/IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL-1 beta/IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat IL-1 beta/IL-1F2 Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed rat splenocytes treated with PMA and calcium ionomycin

Western Blot

0.1 µg/mL
Sample: Recombinant Rat IL-1 beta/IL-1F2 (Catalog # 501-RL)

Rat IL-1 beta/IL-1F2 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Rat IL-1 beta/IL-1F2 Antibody (Catalog # AF-501-NA)
Standard: Recombinant Rat IL-1 beta/IL-1F2 Protein (Catalog # 501-RL)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF501 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

Q63264

Additional IL-1 beta/IL-1F2 Products

Product Documents for Rat IL-1 beta/IL-1F2 Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Rat IL-1 beta/IL-1F2 Biotinylated Antibody

For research use only

Citations
Reviews

Rat IL-1 beta/IL-1F2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF501

Key Product Details

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Species Reactivity

Validated:

Cited:

Applications