mTOR Pathway Antibody Pack
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20207
Key Product Details
Species
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Product Summary for mTOR Pathway Antibody Pack
This pack contains 1 vial each of: AF15371-SP (25 ug), AF15371-SP (0.05 ml), NBP2-24837SS (25 ug), AF4004-SP (25 ug), MAB1665-SP (0.025 ml), NBP1-49674SS (1 mL), HAF008 (1 mL), HAF007 (1 mL), HAF005 (1 mL), HAF017 (0.025 ml).
Product Specifications
Application Notes
See individual datasheets of components for their validated applications
Reactivity Notes
See individual datasheets of components for their validated species
Scientific Data Images for mTOR Pathway Antibody Pack
Detection of Raptor in 293 and NIH3T3 lysates by Western Blot.
Western blot shows lysates of human 293 and mouse NIH3T3 lysate. Raptor was detected by Rabbit Anti-Human Raptor Polyclonal Antibody (Catalog # NBP2-24837) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. This experiment was conducted under reducing conditions.
Detection of Rictor in HeLa by Flow Cytometry.
An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Detection of G beta L (GBL) in Human Prostate by Immunohistochemistry (IHC-P).
G beta L was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human/Mouse/Rat G beta L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4004) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Kit Contents for mTOR Pathway Antibody Pack
- AF15371-SP
- AF4004-SP
- HAF017: Goat IgG HRP-conjugated Antibody
- MAB1665-SP
- HAF007: Mouse IgG Horseradish Peroxidase-conjugated Antibody
- NBP1-49674SS
- NBP1-51645SS
- NBP2-24837SS
- HAF008: Rabbit IgG Horseradish Peroxidase-conjugated Antibody
- HAF005: Rat IgG Horseradish Peroxidase-conjugated Antibody
Formulation, Preparation, and Storage
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Storage
Storage is content dependent.
Background: mTOR Pathway
Additional mTOR Pathway Products
Product Specific Notices for mTOR Pathway Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
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