Mouse Siglec-2/CD22 Antibody

Cell Adhesion Mediated by Siglec‑2/CD22 and Neutral-ization by Mouse Siglec‑2/ CD22 Antibody.

Recombinant Mouse Siglec-2/ CD22 Fc Chimera (Catalog # 2296-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Siglec-2/ CD22 Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Siglec-2/CD22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2296). The ND₅₀ is typically 0.1-0.5 µg/mL.

Siglec‑2/CD22 in Mouse Splenocytes.

Siglec-2/CD22 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Siglec-2/CD22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2296) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Siglec-2/CD22 by Flow Cytometry

High-protein diet reduces villus-resident CD22+ eosinophils. (A) Percent of kilocalories (% kCal) from different macronutrients for diets shown in B. RP = reduced (8%) protein, C = control (18% protein), HP = high (70%) protein, and HF = high fat. (B) Eosinophil frequency in the proximal SI lamina propria (LP) of mice fed diets containing variable macronutrient composition for 15 d, representative of at least 3 experiments with 3 to 4 mice per group. (C and D) Eosinophil frequency (C) and number (D) in the proximal SI LP of mice fed diets containing the indicated percentage of protein (15 d), representative of 2 experiments with 4 to 6 mice per group. (E) Eosinophil frequency in proximal SI LP of mice fed either C, HP, or a diet containing elevated amino acids (“HAA”) in amounts equivalent to those in HP (15 d), representative of 2 experiments with 4 mice per group. (F and G) Representative microscopy images (F) and quantification (G) of eosinophils near crypts and in villi, per region, of the proximal SI of mice fed C or HP diets [red = MBP, blue = DAPI, (Scale bar, 100 μm.) “Duod.” = duodenum, “Jejun.” = jejunum]. (H) Eosinophil frequency in LP of the indicated regions of the gastrointestinal (GI) tract in mice fed C or HP diets for 15 d, representative of 2 experiments with 4 mice/group. (I) Percent of eosinophils that were CD22+ in LP of the indicated regions of the GI tract in mice fed C or HP diet for 15 d, representative of 2 experiments with 4 mice per group. (J) Representative flow cytometry plots showing alpha4 beta7 and CD22 staining in proximal SI LP eosinophils in mice fed C or HP diet for 15 d. Data are presented as mean ± SEM. All data were analyzed by Student’s t test. ∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, and ns = not significant (P > 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38271336), licensed under a CC-BY license. Not internally tested by R&D Systems.