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Mouse Serpin E1/PAI‑1 Antibody

Catalog # AF3828 | R&D Systems, Inc. a Bio-Techne Brand
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AF3828
AF3828-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse Serpin E1/PAI‑1 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI-1
Leu24-Ser402
Accession # AAH54091

Specificity

Detects mouse Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Serpin E1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Serpin E1/PAI‑1 Antibody

Detection of Mouse Serpin E1/PAI-1 antibody by Western Blot.

Detection of Mouse Serpin E1/PAI‑1 by Western Blot.

Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin E1/PAI-1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serpin E1/PAI-1 antibody by Simple WesternTM.

Detection of Mouse Serpin E1/PAI‑1 by Simple WesternTM.

Simple Western lane view shows lysates of mouse placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI-1 at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse Serpin E1/PAI‑1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin E1/PAI‑1, see our available Western blot detection antibodies

Simple Western

12.5 µg/mL
Sample: Mouse placenta tissue

Western Blot

0.25 µg/mL
Sample: Mouse placenta tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 6 publications using AF3828 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E1/PAI-1

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Entrez Gene IDs

5054 (Human); 18787 (Mouse)

Gene Symbol

SERPINE1

UniProt

Product Documents for Mouse Serpin E1/PAI‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Serpin E1/PAI‑1 Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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