Mouse IL-21 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF594
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Bovine, Transgenic Mouse
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Immunocytochemistry
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Capture, ELISA Development, ELISpot Development
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
Pro25-Ser146
Accession # Q9ES17
Pro25-Ser146
Accession # Q9ES17
Specificity
Detects mouse IL-21 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL-15, and recombinant human IL-21 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IL-21 Antibody
IL-21 in Mouse Splenocytes.
IL-21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by IL-21 and Neutralization by Mouse IL-21 Antibody.
Recombinant Mouse IL-21 (Catalog # 594-ML) stimulates proliferation in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594). The ND50 is typically 0.2-2 µg/mL.
Mouse IL-21 ELISA Standard Curve
Recombinant Mouse IL-21 (Catalog # 594-ML) was serially diluted and captured by Goat Anti-Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse IL-21 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed concanavalin A-stimulated mouse splenocytes
Sample: Immersion fixed concanavalin A-stimulated mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL-21 (Catalog # 594-ML)
Sample: Recombinant Mouse IL-21 (Catalog # 594-ML)
Neutralization
Measured by its ability to neutralize IL‑21-induced proliferation in the NK-92 human natural killer lymphoma cell line. The Neutralization Dose (ND50) is typically 0.2-2 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑21.
Mouse IL-21 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF594 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-21
Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid sequence identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gammac) chain appears to play a role in IL-21 R signaling. The IL‑21/IL‑21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.
References
- Parrish-Novak, et al. (2000) Nature 408:57.
- Ozaki, K. et al. (2000) PNAS 97:11439.
- Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
- Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21
Alternate Names
CVID11, IL21, Za11
Gene Symbol
IL21
UniProt
Additional IL-21 Products
Product Documents for Mouse IL-21 Antibody
Product Specific Notices for Mouse IL-21 Antibody
For research use only
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