Mouse IL-18 R alpha /IL-1 R5 Antibody

Name: Mouse IL-18 R alpha /IL-1 R5 Antibody
Brand: R&D Systems
SKU: AF856

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF856

Citations (10)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL-1 R5 Ser19-Gly326 Accession # Q61098
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse IL‑18 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL‑18 R is observed.

View Full Technical Details for Mouse IL-18 R alpha /IL-1 R5 Antibody

Applications for Mouse IL-18 R alpha /IL-1 R5 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑18 R alpha /IL‑1 R5
Flow Cytometry	0.25 µg/10⁶ cells	B6 splenocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Item	Value
Neutralization	Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. Ahn, H.J. et al. (1997) J. Immunol. 159:2125. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse IL-18 R alpha /IL-1 R5 Antibody

	Neutralization IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Mouse IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856). The ND₅₀ is typically 0.2-0.8 µg/mL.
	Flow Cytometry Detection of IL‑18 R alpha /IL‑1 R5 in B6 splenocytes by Flow Cytometry. B6 splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Goat Anti-Mouse IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-18 R alpha/IL-1 R5

Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL18 in vitro, coexpression of IL-18 R alpha and IL18 R beta is required for high affinity binding and IL-18 responsiveness.

Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

Item	Value
Long Name	Interleukin 18 Receptor alpha
Entrez Gene IDs	8809 (Human); 16182 (Mouse)
Alternate Names	CD218a, IL-1 R5, IL-1 Rrp, IL-18 R alpha, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1

Citations for Mouse IL-18 R alpha /IL-1 R5 Antibody (10)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-18 R alpha /IL-1 R5 Antibody include:

Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

JE Howard et al. (2021), IL-18R-mediated HSC quiescence and MLKL-dependent cell death limit hematopoiesis during infection-induced shock Stem Cell Reports, 0(0).
PMID: 34798063
S Kuwahara-O et al. (2017), Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain Neurosci. Lett., 0(0).
PMID: 28373090
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

T Hayakawa et al. (2017), Postnatal changes of interleukin-18 receptor immunoreactivity in neurons of the retrosplenial cortex in wild-type and interleukin-18 knock out mice Okajimas Folia Anat Jpn, 94(3):93-99.
PMID: 29681594
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Nold-Petry CA et al. (2009), Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 0(0).
PMID: 19592492
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Andoh T et al. (2008), Protective effect of IL-18 on kainate- and IL-1beta-induced cerebellar ataxia in mice. J. Immunol., 180(4):2322-8.
PMID: 18250441
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Wiener Z et al. (2008), IL-18 induces a marked gene expression profile change and increased Ccl1 (I-309) production in mouse mucosal mast cell homologs. Int. Immunol., 20(12):1565-73.
PMID: 18940934
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Chandrasekar B et al. (2007), Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation. J. Biol. Chem., 283(7):4200-9.
PMID: 18086672
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Wang X et al. (2006), Disruption of interleukin-18, but not interleukin-1, increases vulnerability to preterm delivery and fetal mortality after intrauterine inflammation. Am. J. Pathol., 169(3):967-76.
PMID: 16936270
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Chaudhry UI et al. (2006), Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth. Cancer Res., 66(21):10497-504.
PMID: 17079471
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Gould MP et al. (2004), Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis. J. Immunol., 172(3):1754-62.
PMID: 14734758

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Mouse IL-18 R alpha /IL-1 R5 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse IL-18 R alpha /IL-1 R5 Antibody

Scientific Data Examples for Mouse IL-18 R alpha /IL-1 R5 Antibody

Neutralization

Flow Cytometry

Formulation, Preparation and Storage

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Background: IL-18 R alpha/IL-1 R5

Citations for Mouse IL-18 R alpha /IL-1 R5 Antibody (10)

Product Documents for Mouse IL-18 R alpha /IL-1 R5 Antibody

Product Datasheets

COA

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