Mouse IGF-I/IGF-1 Biotinylated Antibody

Name: Mouse IGF-I/IGF-1 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF791
Rating: 5 (1 reviews)

Catalog # BAF791 | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (5)

Product Datasheet / COA / SDS

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Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

ELISA Detection (Matched Antibody Pair), Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse IGF-I/IGF-1 Biotinylated Antibody

Immunogen

E. coli-derived recombinant mouse IGF-I/IGF-1
Gly33-Ala102
Accession # Q8CAR0

Specificity

Detects mouse IGF-I/IGF-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human IGF-I/IGF-1 is observed and less than 0.1% cross-reactivity with recombinant mouse IGF-II/IGF-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse IGF-I/IGF-1 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG)

Mouse IGF-I/IGF-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse IGF-I/IGF-1 Antibody (Catalog # MAB791)
Standard: Recombinant Mouse IGF-I/IGF-1 Protein, CF (Catalog # 791-MG)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF791 in the following applications:

ELISA (1 Review)

Published Applications

Read 5 publications using BAF791 in the following applications:

ELISA Development (4 Publications)
ELISA Development (Detection) (1 Publication)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

References

Philippou, A. et al. (2007) In Vivo 21:45.
Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

UniProt

Q8CAR0

Product Documents for Mouse IGF-I/IGF-1 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse IGF-I/IGF-1 Biotinylated Antibody

For research use only

Citations
Reviews

Citations for Mouse IGF-I/IGF-1 Biotinylated Antibody (5)

Showing 1 - 5 of 5 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IGF-I/IGF-1 Biotinylated Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Detection)

Fletcher L et al. (2013-12-21), Spatial distribution of insulin-like growth factor binding protein-2 following hypoxic-ischemic injury. BMC Neurosci, 2013-114(0):158.
PMID: 24359611
Species: Mouse
Sample Types: Serum
Applications: ELISA Development

Mehta HH et al. (2011-06-22), IGFBP-3 is a metastasis suppression gene in prostate cancer. Cancer Res., 2011-071(15):5154-63.
PMID: 21697285
Species: Mouse
Sample Types: Serum
Applications: ELISA Development

Yamada PM et al. (2010-10-06), Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation. Endocrinology, 2010-1151(12):5741-50.
PMID: 20926583
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development

Wang N et al. (2009-04-14), Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases. Mol. Ther., 2009-017(7):1241-9.
PMID: 19367255
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development

Raina K et al. (2007-11-15), Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model. Cancer Res., 2007-167(22):11083-91.
PMID: 18006855

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