Mouse IGF-I/IGF-1 Antibody
Catalog # MAB791 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Mouse IGF-I/IGF-1 Antibody
Immunogen
Gly49-Ala118
Accession # P05017
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse IGF-I/IGF-1 Antibody
Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody.
Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791). The ND50 is typically 0.3-1.5 µg/mL.Applications for Mouse IGF-I/IGF-1 Antibody
Western Blot
Sample: Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG)
Neutralization
Mouse IGF-I/IGF-1 Sandwich Immunoassay
Published Applications
Read 5 publications using MAB791 in the following applications:
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I/IGF-1
Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I/IGF-1 is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I/IGF-1 shares 94% and 99% amino acid (aa) sequence identity with human and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).
References
- Philippou, A. et al. (2007) In Vivo 21:45.
- Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
- Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
- Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
- Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
- Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
- Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
- Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
- LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
- Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Mouse IGF-I/IGF-1 Antibody
Product Specific Notices for Mouse IGF-I/IGF-1 Antibody
For research use only
Citations for Mouse IGF-I/IGF-1 Antibody (5)
Citations are publications that use Bio-Techne products. Selected citations for Mouse IGF-I/IGF-1 Antibody include:
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Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA DevelopmentSteger J et al. (2014-09-25), Insulin-like growth factor 1 is a direct HOXA9 target important for hematopoietic transformation. Leukemia, 2014-029(4):901-8.
PMID: 25252870 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)Fletcher L et al. (2013-12-21), Spatial distribution of insulin-like growth factor binding protein-2 following hypoxic-ischemic injury. BMC Neurosci, 2013-114(0):158.
PMID: 24359611 -
Species: Mouse
Sample Types: Serum
Applications: ELISA DevelopmentMehta HH et al. (2011-06-22), IGFBP-3 is a metastasis suppression gene in prostate cancer. Cancer Res., 2011-071(15):5154-63.
PMID: 21697285 -
Species: Mouse
Sample Types: Serum
Applications: ELISA DevelopmentYamada PM et al. (2010-10-06), Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation. Endocrinology, 2010-1151(12):5741-50.
PMID: 20926583 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA DevelopmentRaina K et al. (2007-11-15), Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model. Cancer Res., 2007-167(22):11083-91.
PMID: 18006855
There are no citations that match your criteria.
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