Mouse IGF-I/IGF-1 Antibody

Name: Mouse IGF-I/IGF-1 Antibody
Brand: R&D Systems
SKU: MAB791

Catalog # MAB791 | R&D Systems, Inc. a Bio-Techne Brand

Citations (5)

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # 126002

Product Summary for Mouse IGF-I/IGF-1 Antibody

Immunogen

E. coli-derived recombinant mouse IGF-I/IGF-1
Gly49-Ala118
Accession # P05017

Specificity

Detects mouse IGF-I/IGF-1 in direct ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human IGF-I/IGF-1, recombinant mouse (rm) IGF-II/IGF-2, rmIGFBP-2, rmIGFBP-5, or rmIGFBP-6 is observed.

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Scientific Data Images for Mouse IGF-I/IGF-1 Antibody

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody.

Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791). The ND₅₀ is typically 0.3-1.5 µg/mL.

Applications for Mouse IGF-I/IGF-1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG)

Neutralization

Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 15 ng/mL Recombinant Mouse IGF‑I.

Mouse IGF-I/IGF-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse IGF-I/IGF-1 Biotinylated Antibody (Catalog # BAF791)
Standard: Recombinant Mouse IGF-I/IGF-1 Protein, CF (Catalog # 791-MG)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 5 publications using MAB791 in the following applications:

ELISA Development (4 Publications)
ELISA Development (Capture) (1 Publication)

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I/IGF-1 is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I/IGF-1 shares 94% and 99% amino acid (aa) sequence identity with human and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

References

Philippou, A. et al. (2007) In Vivo 21:45.
Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

UniProt

P05017

Product Documents for Mouse IGF-I/IGF-1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse IGF-I/IGF-1 Antibody

For research use only

Citations for Mouse IGF-I/IGF-1 Antibody (5)

Showing 1 - 5 of 5 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IGF-I/IGF-1 Antibody include:

Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Steger J et al. (2014-09-25), Insulin-like growth factor 1 is a direct HOXA9 target important for hematopoietic transformation. Leukemia, 2014-029(4):901-8.
PMID: 25252870
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)

Fletcher L et al. (2013-12-21), Spatial distribution of insulin-like growth factor binding protein-2 following hypoxic-ischemic injury. BMC Neurosci, 2013-114(0):158.
PMID: 24359611
Species: Mouse
Sample Types: Serum
Applications: ELISA Development

Mehta HH et al. (2011-06-22), IGFBP-3 is a metastasis suppression gene in prostate cancer. Cancer Res., 2011-071(15):5154-63.
PMID: 21697285
Species: Mouse
Sample Types: Serum
Applications: ELISA Development

Yamada PM et al. (2010-10-06), Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation. Endocrinology, 2010-1151(12):5741-50.
PMID: 20926583
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development

Raina K et al. (2007-11-15), Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model. Cancer Res., 2007-167(22):11083-91.
PMID: 18006855

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