Mouse IGF-I/IGF-1 Antibody
Catalog # AF791 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Mouse IGF-I/IGF-1 Antibody
Immunogen
Gl33-Ala102
Accession # Q8CAR0
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse IGF-I/IGF-1 Antibody
Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody.
Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND50 is typically 0.2-1.0 µg/mL.IGF-I/IGF-1 in Mouse Embryo.
IGF-I/IGF-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse IGF-I/IGF-1 Antibody
Immunohistochemistry
Sample: Immersion fixed frozen sections of mouse embryo (E13)
Western Blot
Sample: Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG)
Neutralization
Reviewed Applications
Read 2 reviews rated 4 using AF791 in the following applications:
Published Applications
Read 21 publications using AF791 in the following applications:
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I/IGF-1
Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).
References
- Philippou, A. et al. (2007) In Vivo 21:45.
- Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
- Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
- Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
- Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
- Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
- Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
- Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
- LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
- Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Mouse IGF-I/IGF-1 Antibody
Product Specific Notices for Mouse IGF-I/IGF-1 Antibody
For research use only
Citations for Mouse IGF-I/IGF-1 Antibody (21)
Citations are publications that use Bio-Techne products. Selected citations for Mouse IGF-I/IGF-1 Antibody include:
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Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotMiyagawa et al. (2023-06-20), Osteoclast-derived IGF1 induces RANKL production in osteocytes and contributes to Pagetic lesion formation JCI insight
PMID: 37338990 -
Species: Mouse
Sample Types: In Vivo
Applications: In VivoJ Wang et al. (2022-12-28), Bone marrow-derived IGF-1 orchestrates maintenance and regeneration of the adult skeleton Proceedings of the National Academy of Sciences of the United States of America, 2022-1120(1):e2203779120.
PMID: 36577075 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC/IFZ Liu et al. (2022-12-08), Single-cell transcriptome analyses reveal microglia types associated with proliferative retinopathy JCI Insight, 2022-17(23).
PMID: 36264636 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCR Ronzano et al. (2021-09-01), Microglia-neuron interaction at nodes of Ranvier depends on neuronal activity through potassium release and contributes to remyelination Nature Communications, 2021-012(1):5219.
PMID: 34471138 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotHJ Kok et al. (2021-07-19), Muscle insulin-like growth factor-I modulates murine craniofacial bone growth European cells & materials, 2021-041(0):72-89.
PMID: 34279041 -
Species: Mouse
Sample Types: Whole Cells,Whole Tissue
Applications: Neutralization,IHC-PM Inoue et al. (2019-03-20), Forceful mastication activates osteocytes and builds a stout jawbone Sci Rep, 2019-09(1):4404.
PMID: 30890758 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCV Bellver-La et al. (2019-01-31), Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury Nat Commun, 2019-010(1):518.
PMID: 30705270 -
Species: Mouse
Sample Types: Cell Lysates,Whole Cells
Applications: Neutralization,Western BlotLE Hillers et al. (2018-10-12), Obesity-Activated Adipose-Derived Stromal Cells Promote Breast Cancer Growth and Invasion Neoplasia, 2018-120(11):1161-1174.
PMID: 30317122 -
Species: Mouse
Sample Types: Tissue Micorarray
Applications: IHCJW Park et al. (2018-05-03), AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development Mol. Cancer Res., 2018-00(0).
PMID: 29724814 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotT Gao et al. (2018-04-03), Systemic IGF-1 gene delivery by rAAV9 improves spontaneous autoimmune peripheral polyneuropathy (SAPP) Sci Rep, 2018-08(1):5408.
PMID: 29615658 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCZ Zuo et al. (2017-02-09), Immunization with Bacillus Calmette-Gu�rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain Neurobiol. Dis, 2017-0101(0):27-39.
PMID: 28189498 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationK Kimura et al. (2017-01-05), Combined Effects of Androgen and Growth Hormone on Osteoblast Marker Expression in Mouse C2C12 and MC3T3-E1 Cells Induced by Bone Morphogenetic Protein J Clin Med, 2017-06(1).
PMID: 28067796 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationC Zhao et al. (2016-01-14), Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism Sci Rep, 2016-06(0):19404.
PMID: 26762853 -
Species: Rat
Sample Types: In Vivo
Applications: In VivoHwang D et al. (2014-09-17), Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling. J Neurosci, 2014-034(38):12788-800.
PMID: 25232115 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotBrisson et al. (2014-02-25), Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength. Am J Physiol Endocrinol Metab, 2014-0306(8):E965-74.
PMID: 24569593 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrKoronyo-Hamaoui M et al. (2009-09-24), Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9. J. Neurochem., 2009-0111(6):1409-24.
PMID: 19780903 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrScolnick JA et al. (2008-03-27), Role of IGF signaling in olfactory sensory map formation and axon guidance. Neuron, 2008-057(6):847-57.
PMID: 18367086 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationKubota Y et al. (2008-01-15), Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat. Biochem. Biophys. Res. Commun., 2008-0368(1):81-7.
PMID: 18201559 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCButovsky O et al. (2006-07-24), Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1. Proc. Natl. Acad. Sci. U.S.A., 2006-0103(31):11784-9.
PMID: 16864778 -
Species: Mouse
Sample Types: Whole Cells,Whole Tissue
Applications: ICC,IHC-FrButovsky O et al. (2006-03-23), Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis. J. Clin. Invest., 2006-0116(4):905-15.
PMID: 16557302 -
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization,ICCButovsky O et al. (2005-11-16), Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells. Mol. Cell. Neurosci., 2005-131(1):149-60.
PMID: 16297637
There are no citations that match your criteria.
Customer Reviews for Mouse IGF-I/IGF-1 Antibody (2)
Customer Images
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Application: Western BlotSample Tested: Purified proteinSpecies: MouseVerified Customer | Posted 04/18/2019Could not detect IGF-1 when I used liver samples from mice.
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Application: Western BlotSample Tested: See PMID 23407451Species: MouseVerified Customer | Posted 01/09/2015
There are no reviews that match your criteria.
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