Mouse Clusterin Antibody

Name: Mouse Clusterin Antibody
Brand: R&D Systems
SKU: AF2747
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2747

Citations (5)

(2)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse Clusterin Glu22-Glu448 Accession # Q06890
Applications:	ELISA Capture, Immunohistochemistry, Immunoprecipitation, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse Clusterin in ELISAs and Western blots. In sandwich ELISAs, approximately 5% cross-reactivity with recombinant human Clusterin is observed.

View Full Technical Details for Mouse Clusterin Antibody

Applications for Mouse Clusterin Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Clusterin (Catalog # 2747-HS)
Immunohistochemistry	5-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Mouse Clusterin (Catalog # 2747‑HS), see our available Western blot detection antibodies

Mouse Clusterin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse Clusterin Biotinylated Antibody (Catalog # BAF2747)

Standard: Recombinant Mouse Clusterin Protein, CF (Catalog # 2747-HS)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse Clusterin Antibody

	Immunohistochemistry Clusterin in Mouse Liver. Clusterin was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Immunohistochemistry Clusterin in Mouse Intestine. Clusterin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Clusterin

Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40,40, is a secreted multifunctional protein that was named for its ability to induce cellular clustering. It binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis (1, 2). Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development (3). Mouse Clusterin is synthesized as a precursor that contains two coiled coil domains, two nuclear localization signals (NLS), and one heparin binding domain (3-6). Intracellular cleavages of the precursor remove the signal peptide and generate comparably sized alpha and beta chains which are secreted as an 80 kDa N-glycosylated disulfide-linked heterodimer (7, 8). Mature mouse Clusterin shares 77% and 93% amino acid sequence identity with human and rat Clusterin, respectively. High μg/mL concentrations of Clusterin circulate predominantly as a component of high density lipoprotein particles, and these are internalized and degraded through interactions with LRP-2/Megalin (9, 10). In human, an alternately spliced 50 kDa isoform of Clusterin (nCLU) lacks the signal peptide and remains intracellular (5, 11). This molecule is neither glycosylated nor cleaved into alpha and beta chains (11). In the cytoplasm, nCLU destabilizes the actin cytoskeleton and inhibits NF kappa B activation (12, 13). Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus where it interacts with Ku70 and promotes apoptosis (5, 11). This function contrasts with the cytoprotective effect of secreted Clusterin (14). During colon cancer tumor progression there is a downregulation of the intracellular form and an upregulation of the glycosylated secreted form (11).

Item	Value
References	Carver, J.A. et al. (2003) IUBMB Life 55:661. Shannan, B. et al. (2006) Cell Death Differ. 13:12. French, L.E. et al. (1993) J. Cell Biol. 122:1119. Lee, K.H. et al. (1993) Biochem. Biophys. Res. Commun. 194:1175. Leskov, K.S. et al. (2003) J. Biol. Chem. 278:11590. Pankhurst, G.J. et al. (1998) Biochemistry 37:4823. Burkey, B.F. et al. (1991) J. Lipid. Res. 32:1039. de Silva, H.V. et al. (1990) J. Biol. Chem. 265:14292. Jenne, D.E. et al. (1991) J. Biol. Chem. 266:11030. Kounnas, M.Z. et al. (1995) J. Biol. Chem. 270:13070. Pucci, S. et al. (2004) Oncogene 23:2298. Moretti, R. M. et al. (2007) Cancer Res. 67:10325. Santilli, G. et al. (2003) J. Biol. Chem. 278:38214. Trougakos, I.P. et al. (2004) Cancer Res. 64:1834.
Entrez Gene IDs	1191 (Human); 12759 (Mouse)
Alternate Names	40, , , , , , , , , , , , , , , , , , , , , , , ,

Citations for Mouse Clusterin Antibody (5)

Showing 1 - 5 of 5 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse Clusterin Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

HW Cheng et al. (2022), Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function Nature Communications, 13(1):2027.
PMID: 35440118
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

DM Go et al. (2021), Regulation of Helicobacter-induced gastritis by programmed death ligand 1-expressing classical dendritic cells Cellular and Molecular Gastroenterology and Hepatology, 0(0).
PMID: 33894424
Species: Mouse
Sample Types: Whole Cells
Applications: ICC/IF

K Murata et al. (2020), Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells Cell Stem Cell, 0(0).
PMID: 32084390
Afanasyeva M et al. (2014), Clusterin is a potential lymphotoxin beta receptor target that is upregulated and accumulates in germinal centers of mouse spleen during immune response. PLoS ONE, 9(5):e98349.
PMID: 24865838
Species: Human
Sample Types: Urine
Applications: ELISA Development

Dieterle F et al. (2010), Urinary clusterin, cystatin C, beta2-microglobulin and total protein as markers to detect drug-induced kidney injury. Nat. Biotechnol., 28(5):463-9.
PMID: 20458316

There are no citations that match your criteria.

Showing 1 - 5 of 5 citations Showing All

Customer Reviews for Mouse Clusterin Antibody (2)

5 out of 5

2 customer ratings

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 2 of 2 reviews Showing All

Filter By:

Mouse Clusterin Antibody

Name: Anonymous

Application: WB

Sample Tested: Kidney tissue

Species: Mouse

Verified Customer | Posted 06/14/2017

- Reconstituted SP size in 125 uL of PBS- 10% gel; 20ug of total protein- Diluted primary antibody 1:500 in 5% milk overnight- Diluted secondary antibody (goat) 1:5000 in 5% milk (non-R&D) for 45 minutes- Two bands seen at
Mouse Clusterin Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Heparin Plasma

Species: Mouse

Verified Customer | Posted 08/11/2016

There are no reviews that match your criteria.

Showing 1 - 2 of 2 reviews Showing All

Product Documents for Mouse Clusterin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Mouse Clusterin Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Mouse Clusterin Antibody

Mouse Clusterin Sandwich Immunoassay

Scientific Data Examples for Mouse Clusterin Antibody

Immunohistochemistry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Clusterin

Citations for Mouse Clusterin Antibody (5)

Customer Reviews for Mouse Clusterin Antibody (2)

Customer Images

Mouse Clusterin Antibody

Mouse Clusterin Antibody

Product Documents for Mouse Clusterin Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected