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Mouse CD83 Antibody

Bio-Techne includes R&D Systems | Catalog # AF1437

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AF1437
AF1437-SP

Key Product Details

Species Reactivity

Mouse

Applications

Adhesion Blockade, CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse CD83 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD83
Met22-Ala134
Accession # O88324

Specificity

Detects mouse CD83 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CD83 Antibody

CD83 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD83 in Mouse Splenocytes.

CD83 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD83 antibody in Mouse Dendritic Cells by Immunocytochemistry (ICC).

CD83 in Mouse Dendritic Cells.

CD83 was detected in immersion fixed LPS-stimulated mouse dendritic cells using Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse CD83 Antibody

Application
Recommended Usage

Adhesion Blockade

The adhesion of human monocyte-derived dendritic cells (5 x 104 cells/well) to immobilized Recombinant Mouse CD83 Fc Chimera (Catalog # 1437-CD, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 40 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: LPS-treated mature mouse dendritic cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes and dendritic cells

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD83 Fc Chimera (Catalog # 1437-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF1437 in the following applications:

Published Applications

Read 2 publications using AF1437 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD83

Mouse CD83 is a 30‑35 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 114 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and a 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4). Relative to mouse, human CD83 has an 11 aa insertion in its extracellular domain and is expressed as a 45‑55 kDa protein (1, 4, 5, 6). No alternate splice variants have been reported for mouse. In human, however, one soluble splice form has been reported and proteolytic processing is suggested to generate a second circulating isoform (6, 7). Notably, although soluble CD83 has the potential to exist as either a monomer or disulfide-linked dimer, both show immunosuppressive activity (4, 8, 9). Membrane CD83, by contrast, is immunostimulatory (10). CD83 is a primary marker for dendritic cells (3, 5, 6). It is also found on B cells (6, 11), neutrophils (12), monocytes and macrophages (13). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on monocytes (3). The function of CD83 is only now becoming clear. As noted, membrane-immobilized CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (14). On monocytes, CD83 may also drive monocytes into a fibrocyte phenotype (14). And a lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (15).

References

  1. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
  2. Fujimoto, Y. and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
  3. Scholler, N. et. al. (2001) J. Immunol. 166:3865.
  4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
  5. Zhou, L-J. et. al. (1992) J. Immunol. 149:735.
  6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
  7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
  8. Kotzor, N. et al. (2004) Immunobiology 209:129.
  9. Zinser, E. et al. (2006) Immunobiology 21:449.
  10. Hirano, N. et al. (2006) Blood 107:1528.
  11. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
  12. Yamashiro, S. et al. (2000) Blood 96:3958.
  13. Cao, W. et al. (2005) Biochem. J. 385:85.
  14. Scholler, N. et al. (2002) J. Immunol. 168:2599.
  15. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.

Alternate Names

BL11, CD83, HB15

Entrez Gene IDs

9308 (Human); 12522 (Mouse)

Gene Symbol

CD83

UniProt

Additional CD83 Products

Product Documents for Mouse CD83 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD83 Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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