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Recombinant Mouse CD83 Fc Chimera Protein, CF

Catalog # 1437-CD | R&D Systems, Inc. a Bio-Techne Brand
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1437-CD-050

Key Product Details

Source

Sf 21 (stably transfected)

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse CD83 protein
Mouse CD83
(Met22 - Ala134)
Accession #NP_033986
DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met22

Predicted Molecular Mass

39.1 kDa (monomer)

SDS-PAGE

52 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of human monocyte-derived dendritic cells.
When 5 x 104 cells/well are added to mouse CD83/Fc Chimera coated plates (2.5 µg/mL with 100 µL/well), approximately 50-75% will adhere after 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

1437-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD83

Mouse CD83 is a 30 - 35 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 114 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and a 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4). Relative to mouse, human CD83 has an 11 aa insertion in its extracellular domain and is expressed as a 45 - 55 kDa protein (1, 4, 5, 6). No alternate splice variants have been reported for mouse. In human, however, one soluble splice form has been reported and proteolytic processing is suggested to generate a second circulating isoform (6, 7). Notably, although soluble CD83 has the potential to exist as either a monomer or disulfide-linked dimer, both show immunosuppressive activity (4, 8, 9). Membrane CD83, by contrast, is immunostimulatory (10). CD83 is a primary marker for dendritic cells (3, 5, 6). It is also found on B cells (6, 11), neutrophils (12), monocytes and macrophages (13). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on monocytes (3). The function of CD83 is only now becoming clear. As noted, membrane-immobilized CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (14). On monocytes, CD83 may also drive monocytes into a fibrocyte phenotype (14). And a lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (15).

References

  1. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
  2. Fujimoto, Y. and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
  3. Scholler, N. et. al. (2001) J. Immunol. 166:3865.
  4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
  5. Zhou, L-J. et. al. (1992) J. Immunol. 149:735.
  6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
  7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
  8. Kotzor, N. et al. (2004) Immunobiology 209:129.
  9. Zinser, E. et al. (2006) Immunobiology 21:449.
  10. Hirano, N. et al. (2006) Blood 107:1528.
  11. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
  12. Yamashiro, S. et al. (2000) Blood 96:3958.
  13. Cao, W. et al. (2005) Biochem. J. 385:85.
  14. Scholler, N. et al. (2002) J. Immunol. 168:2599.
  15. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.

Alternate Names

BL11, CD83, HB15

Entrez Gene IDs

9308 (Human); 12522 (Mouse)

Gene Symbol

CD83

UniProt

Additional CD83 Products

Product Documents for Recombinant Mouse CD83 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse CD83 Fc Chimera Protein, CF

For research use only

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