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Mouse CD3 epsilon Alexa Fluor™ Plus 680-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB484AFP680

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FAB484AFP680-100UG
Conjugate
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunoprecipitation, Cell depletion, T Cell Stimulation, CyTOF-reported

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Hamster IgG Clone # 145-2C11

Product Specifications

Specificity

Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Applications

Application
Recommended Usage

Cell depletion

Optimal dilution of this antibody should be experimentally determined.

CyTOF-reported

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

T Cell Stimulation

Optimal dilution of this antibody should be experimentally determined.

Background: CD3 epsilon

CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.4 The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.1 145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.6, 7

References

  1. Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374.
  2. Portoles, P. et al. (1989) J. Immunol. 142:4169.
  3. Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703.
  4. Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583.
  5. Small, M. et al. (1994) J. Immunol. Meth. 167:103.
  6. Alegre, M.L. et al. (1991) J. Immunol. 146:1184.
  7. Hendrickson, M. et al. (1995) Transplantation 60:828.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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