Recombinant Mouse alpha-Fetoprotein/AFP protein was serially diluted 2-fold and captured by Rat Anti-Mouse alpha-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha-Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Mouse alpha-Fetoprotein/AFP Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Mouse alpha‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse alpha-Fetoprotein/AFP DuoSet ELISA Kit (Catalog # DY5369-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Fetoprotein/AFP

AFP ( alpha-Fetoprotein) is a 69‑73 kDa member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids (aa) in length. It contains three albumin domains (aa 20‑201, 208‑393 and 400‑591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37 kDa form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa 19‑605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Gene Symbol

AFP

UniProt

P02772

Additional alpha-Fetoprotein/AFP Products

Product Documents for Mouse alpha-Fetoprotein/AFP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse alpha-Fetoprotein/AFP Antibody

For research use only

Citations
Reviews

Mouse alpha-Fetoprotein/AFP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5369

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all alpha-Fetoprotein/AFP Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse alpha-Fetoprotein/AFP Antibody

Mouse alpha-Fetoprotein/AFP ELISA Standard Curve.