Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all alpha-Fetoprotein/AFP Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse alpha‑Fetoprotein/AFP
Lys19-Val605
Accession # P02772

Specificity

Detects mouse alpha‑Fetoprotein/AFP in Western blots. In Western blots, approximately 10% cross-reactivity with human alpha-Fetoprotein is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse alpha-Fetoprotein/AFP Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse alpha‑Fetoprotein/AFP

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Fetoprotein/AFP

AFP ( alpha-Fetoprotein)is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids (aa) in length. It contains three albumin domains (aa 20-201, 208-393 and 400-591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37 kDa form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa 19-605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Gene Symbol

AFP

UniProt

P02772

Additional alpha-Fetoprotein/AFP Products

Product Documents for Mouse alpha-Fetoprotein/AFP Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse alpha-Fetoprotein/AFP Biotinylated Antibody

For research use only

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