Human VEGFR1/Flt-1 Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # MAB321
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Flow Cytometry, CyTOF-ready
Cited:
Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Cell Culture, ELISA Development, Microarray
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 49560
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
Ser27-His687
Accession # NP_001153392
Ser27-His687
Accession # NP_001153392
Specificity
Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGFR1, recombinant human (rh) VEGFR2, rhVEGFR3, or rhVEGFR4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human VEGFR1/Flt-1 Antibody
Detection of VEGFR1/Flt‑1 in HUVEC Human Cells by Flow Cytometry.
HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human VEGFR1/Flt-1 Monoclonal Antibody (Catalog # MAB321, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Applications for Human VEGFR1/Flt-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells
Sample: HUVEC human umbilical vein endothelial cells
Western Blot
1 µg/mL
Sample: Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL)
under non-reducing conditions only
Sample: Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL)
under non-reducing conditions only
Reviewed Applications
Read 2 reviews rated 5 using MAB321 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGFR1/Flt-1
Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.
Long Name
Vascular Endothelial Growth Factor Receptor 1
Alternate Names
Flt-1, FLT1, FRT, VEGF R1, VEGFR-1
Gene Symbol
FLT1
UniProt
Additional VEGFR1/Flt-1 Products
Product Documents for Human VEGFR1/Flt-1 Antibody
Product Specific Notices for Human VEGFR1/Flt-1 Antibody
For research use only
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