Detects human TRAIL R4/TNFRSF10D in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, TRAIL R3, rhTRAIL, rhTNF‑ alpha, and rhTNF-beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human TRAILR4/TNFRSF10D APC-conjugated Antibody

Detection of TRAIL R4/TNFRSF10D in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human TRAIL R4/TNFRSF10D APC-conjugated Monoclonal Antibody (Catalog # FAB633A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRAILR4/TNFRSF10D APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood granulocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAILR4/TNFRSF10D

Human TRAIL R4, also called Decoy Receptor 2 (DcR2) and TRUNND (TRAIL Receptor with a Truncated Death Domain), is a type I, TNF R family transmembrane protein, which is a receptor for TRAIL (APO2 Ligand). In the TNF superfamily nomenclature, TRAIL R4 is designated as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

References

Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
Pan, G. et al. (1998) FEBS lett 424:41.
Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
Degli-Esposti, M.A. et al. (1997) Immunity 7:813.

Long Name

TRAIL Receptor 4

Alternate Names

CD264, DcR2, TNFRSF10D, TRAIL R4

Entrez Gene IDs

8793 (Human)

Gene Symbol

TNFRSF10D

UniProt

Q9UBN6

Additional TRAILR4/TNFRSF10D Products

Product Documents for Human TRAILR4/TNFRSF10D APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TRAILR4/TNFRSF10D APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human TRAILR4/TNFRSF10D APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB633A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications