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Human TRAIL R2/TNFRSF10B Biotinylated Antibody
Catalog # BAF631 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Summary for Human TRAIL R2/TNFRSF10B Biotinylated Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
Ala54-Glu182
Accession # Q6FH58
Ala54-Glu182
Accession # Q6FH58
Specificity
Detects human TRAIL R2/TNFRSF10B in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) TRAIL R1 is observed and less than 1% cross‑reactivity with rhTRAIL R3 and rhTRAIL R4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human TRAIL R2/TNFRSF10B Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 631-T2)
Sample: Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 631-T2)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 4 publications using BAF631 in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R2/TNFRSF10B
Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.
Product Documents for Human TRAIL R2/TNFRSF10B Biotinylated Antibody
Product Specific Notices for Human TRAIL R2/TNFRSF10B Biotinylated Antibody
For research use only
Citations for Human TRAIL R2/TNFRSF10B Biotinylated Antibody (4)
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Citations are publications that use Bio-Techne products. Selected citations for Human TRAIL R2/TNFRSF10B Biotinylated Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryGatti L et al. (2013-12-17), Improved apoptotic cell death in drug-resistant non-small-cell lung cancer cells by tumor necrosis factor-related apoptosis-inducing ligand-based treatment. J Pharmacol Exp Ther, 2013-1348(3):360-71.
PMID: 24345465 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryReddy RM et al. (2007-01-01), Rapid and profound potentiation of Apo2L/TRAIL-mediated cytotoxicity and apoptosis in thoracic cancer cells by the histone deacetylase inhibitor Trichostatin A: the essential role of the mitochondria-mediated caspase activation cascade. Apoptosis, 2007-012(1):55-71.
PMID: 17136498 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryTsai WS et al. (2006-12-01), Enhancement of Apo2L/TRAIL-mediated cytotoxicity in esophageal cancer cells by cisplatin. Mol. Cancer Ther., 2006-15(12):2977-90.
PMID: 17172403 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryMouzakiti A et al. (2003-07-01), Regulation of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Burkitt's lymphoma cell lines. Br. J. Haematol., 2003-0122(1):61-9.
PMID: 12823346
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