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Human TRAIL R2/TNFRSF10B Antibody

Catalog # AF631 | R&D Systems, Inc. a Bio-Techne Brand
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AF631
AF631-SP

Key Product Details

Species Reactivity

Human

Applications

Agonist Activity, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human TRAIL R2/TNFRSF10B Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
Ala54-Glu182
Accession # Q6FH58

Specificity

Detects human TRAIL R2/TNFRSF10B in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TRAIL R2/TNFRSF10B Antibody

Detection of Human TRAIL R2/TNFRSF10B antibody by Western Blot.

Detection of Human TRAIL R2/TNFRSF10B by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF631) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for TRAIL R2/TNFRSF10B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human TRAIL R2/TNFRSF10B Antibody

Application
Recommended Usage

Agonist Activity

Measured by its ability to induce apoptosis on Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 7.00 - 70.0 ng/mL.

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 28 publications using AF631 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References

  1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
  2. Walczak, H. et al. (1997) EMBO J. 16:5386.
  3. Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Gene Symbol

TNFRSF10B

UniProt

Product Documents for Human TRAIL R2/TNFRSF10B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TRAIL R2/TNFRSF10B Antibody

For research use only

Citations for Human TRAIL R2/TNFRSF10B Antibody (28)

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