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Human TNF-alpha Antibody

Cytotoxicity Induced by TNF‑ alpha  and Neutralization by Human TNF-alpha  Antibody.
(2)
TNF-a antibody in Human PBMCs by Immunocytochemistry (ICC).
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AF-210-NA
AF-210-SP

Key Product Details

Species Reactivity: Human
Immunogen: E. coli-derived recombinant human TNF-alpha
Val77-Leu233
Accession # P01375
Applications: Block/Neutralize, Immunocytochemistry/Immunofluorescence, Western Blot
Label: Unconjugated
Source: Polyclonal Goat IgG
Specificity:

Detects human TNF‑ alpha in direct ELISAs and Western blots.

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Citations (23)

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Customer Reviews (3)

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  • Human TNF-alpha Antibody
    Name: Anonymous
    Application: IHC
    Sample Tested: Adult brain
    Species: Human
    Verified Customer | Posted 04/13/2018
    Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287Used at 10ug/ml.Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).
    Human TNF-alpha  Antibody Immunohistochemistry AF-210-NA
  • Human TNF-alpha Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Citrate Plasma,EDTA Plasma,Heparin Plasma
    Species: Human
    Verified Customer | Posted 01/18/2018
  • Human TNF-alpha Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    This antibody was used with MAB610 to build an ELISA to measure TNFa. This antibody was used as the detection while MAB610 was used as the capture.
    Human TNF-alpha  Antibody ELISA AF-210-NA

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