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Human TGF-beta  RIII Antibody

Bio-Techne includes R&D Systems | Catalog # AF-242-PB

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AF-242-PB
AF-242-SP

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human TGF-beta  RIII Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF-beta  RIII
Gly21-Asp781
Accession # AAA67061

Specificity

Detects human TGF-beta  RIII in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) TGF-beta  RIII is observed, and less than 1% cross-reactivity with recombinant human (rh) TGF-beta  RI, rhTGF-beta  RII, rhTGF-beta  RIIB, and rmTGF-beta  RI  is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TGF-beta  RIII Antibody

TGF-beta RIII antibody in Human Placenta Tissue by Immunohistochemistry (IHC-P).

TGF-beta RIII in Human Placenta Tissue.

TGF-beta RIII was detected in immersion fixed paraffin-embedded sections of human placenta tissue using Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on syncytiotrophoblast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of TGF-beta RIII in MCF-7 Human Cell Line by Flow Cytometry.

MCF-7 human breast adenocarcinoma cell line was stained with Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB, filled histogram) or control antibody (AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (F0107).
Detection of TGF-beta  RIII antibody in PC-3 Human Cell Line antibody by Flow Cytometry.

Detection of TGF-beta RIII in PC-3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109).

Applications for Human TGF-beta  RIII Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MCF-7 human breast adenocarcinoma cell line and PC‑3 human prostate cancer cell line

Immunohistochemistry

1-15 µg/mL
Sample: Fixed paraffin-embedded sections of human placenta tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human TGF-beta  RIII (Catalog # 242-R3)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using AF-242-PB in the following applications:

Published Applications

Read 18 publications using AF-242-PB in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RIII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta.

The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Long Name

Transforming Growth Factor beta Receptor III

Alternate Names

Betaglycan, BGCAN, TBRIII, TGF-bRIII, TGFbetaRIII, TGFBR3

Entrez Gene IDs

7049 (Human); 21814 (Mouse); 29610 (Rat)

Gene Symbol

TGFBR3

UniProt

Additional TGF-beta RIII Products

Product Documents for Human TGF-beta  RIII Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TGF-beta  RIII Antibody

For research use only

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