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Human TGF-beta  RIII Antibody

Bio-Techne includes R&D Systems | Catalog # MAB2421

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MAB2421
MAB2421-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 31308

Product Summary for Human TGF-beta  RIII Antibody

Immunogen

Murine myeloma cell line, NS0-derived recombinant human TGF-beta RIII
Gly21-Asp781
Accession # AAA67061

Specificity

Detects human TGF-beta RIII in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) TGF-beta RI, RII, RIIb, recombinant mouse (rm) TGF-beta  RIII, rhTGF-beta 1, beta1.2, beta 2, beta 3, rhLatent TGF-beta 1, or rhLAP is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human TGF-beta  RIII Antibody

Application
Recommended Usage

Human TGF-beta RIII Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human TGF-beta  RIII Biotinylated Antibody (Catalog # BAF242)
  • Standard: Recombinant Human TGF-beta RIII Protein (Catalog # 242-R3)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3 using MAB2421 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RIII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta. The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Long Name

Transforming Growth Factor beta Receptor III

Alternate Names

Betaglycan, BGCAN, TBRIII, TGF-bRIII, TGFbetaRIII, TGFBR3

Entrez Gene IDs

7049 (Human); 21814 (Mouse); 29610 (Rat)

Gene Symbol

TGFBR3

UniProt

Additional TGF-beta RIII Products

Product Documents for Human TGF-beta  RIII Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TGF-beta  RIII Antibody

For research use only

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