Human TGF-beta RI/ALK‑5 Antibody
Catalog # AF3025 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Human TGF-beta RI/ALK‑5 Antibody
Immunogen
Leu34-Glu125
Accession # P36897
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human TGF-beta RI/ALK‑5 Antibody
Detection of Human TGF-beta RI/ALK‑5 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF-beta RI/ALK-5 at approximately 50 & 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.TGF-beta RI/ALK‑5 in Human PBMCs.
TGF-beta RI/ALK-5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human TGF-beta RI/ALK‑5 Antibody
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TGF‑ beta RI/ALK‑5 Monoclonal Antibody (Catalog # MAB3025).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Immunocytochemistry
Sample: Immersion fixed human peripheral blood mononuclear cells
Western Blot
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line
Reviewed Applications
Read 2 reviews rated 5 using AF3025 in the following applications:
Formulation, Preparation and Storage
Purification
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Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RI/ALK-5
Product Documents for Human TGF-beta RI/ALK‑5 Antibody
Product Specific Notices for Human TGF-beta RI/ALK‑5 Antibody
For research use only
Citations for Human TGF-beta RI/ALK‑5 Antibody (9)
Citations are publications that use Bio-Techne products. Selected citations for Human TGF-beta RI/ALK‑5 Antibody include:
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Species: Mouse
Sample Types: Tissue Lysates
Applications: Western BlotH Zhang et al. (2022-12-10), Mitochondrial dysfunction induces ALK5-SMAD2-mediated hypovascularization and arteriovenous malformations in mouse retinas Nature Communications, 2022-113(1):7637.
PMID: 36496409 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotW Cheng et al. (2021-12-08), Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma Nature Communications, 2021-112(1):7142.
PMID: 34880251 -
Species: Human
Sample Types: Protein
Applications: Western BlotB Nam et al. (2020-12-21), TGF&beta1 Suppressed Matrix Mineralization of Osteoblasts Differentiation by Regulating SMURF1-C/EBP&beta-DKK1 Axis International Journal of Molecular Sciences, 2020-121(24).
PMID: 33371439 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotB Wang et al. (2019-02-07), POH1 contributes to hyperactivation of TGF-? signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-? receptors and caveolin-1 EBioMedicine, 2019-00(0).
PMID: 30745168 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotYing Chen et al. (2017-08-17), MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells. British Journal of Cancer, 2017-00(0):1532-1827.
PMID: 28817834 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotGore B et al. (2014-06-23), Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension. PLoS ONE, 2014-09(6):e100310.
PMID: 24956016 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotLei Z et al. (2014-02-20), MiR-142-3p represses TGF-beta-induced growth inhibition through repression of TGFbetaR1 in non-small cell lung cancer. FASEB J, 2014-028(6):2696-704.
PMID: 24558198 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,ICCYao Y et al. (2006-09-27), Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells. Cardiovasc. Res., 2006-074(2):279-89.
PMID: 17074310 -
Species: Bovine
Sample Types: Tissue Homogenates
Applications: Western BlotHaynes LP et al. (2006-03-01), Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta. Proteomics, 2006-06(6):1822-32.
PMID: 16470652
There are no citations that match your criteria.
Customer Reviews for Human TGF-beta RI/ALK‑5 Antibody (2)
Customer Images
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Application: Affinity PurificationSample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell lineSpecies: MouseVerified Customer | Posted 05/07/2019
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Application: Western BlotSample Tested: Liver cellsSpecies: HumanVerified Customer | Posted 08/20/2018
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