Human TGF-beta RI/ALK‑5 Antibody

Name: Human TGF-beta RI/ALK‑5 Antibody
Brand: R&D Systems
SKU: AF3025
Rating: 5 (2 reviews)

Catalog # AF3025 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (9)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human TGF-beta RI/ALK‑5 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF-beta RI/ALK-5
Leu34-Glu125
Accession # P36897

Specificity

Detects human TGF-beta RI/ALK-5 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse TGF‑ beta RI is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TGF-beta RI/ALK‑5 Antibody

Detection of Human TGF-beta RI/ALK‑5 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF-beta RI/ALK-5 at approximately 50 & 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TGF-beta RI/ALK‑5 in Human PBMCs.

TGF-beta RI/ALK-5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human TGF-beta RI/ALK‑5 Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TGF‑ beta RI/ALK‑5 Monoclonal Antibody (Catalog # MAB3025).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells

Western Blot

0.1-0.3 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using AF3025 in the following applications:

Affinity Purification (1 Review)
Western Blot (1 Review)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RI/ALK-5

TGF-beta RI, also known as activin-like kinase 5 (ALK-5), is a 55 kDa type I transmembrane serine/threonine receptor kinase. It is ubiquitously expressed and forms a functional heterotetrameric signaling TGF-beta receptor complex with TGF-beta RII. The amino acid sequence of human TGF-beta RI extracellular domain is 91%, 90%, 91%, and 93% identical to that of porcine, mouse, bovine, and rat TGF-beta RI, respectively.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

P36897

Product Documents for Human TGF-beta RI/ALK‑5 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TGF-beta RI/ALK‑5 Antibody

For research use only

Citations
Reviews

Citations for Human TGF-beta RI/ALK‑5 Antibody (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human TGF-beta RI/ALK‑5 Antibody include:

Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot

H Zhang et al. (2022-12-10), Mitochondrial dysfunction induces ALK5-SMAD2-mediated hypovascularization and arteriovenous malformations in mouse retinas Nature Communications, 2022-113(1):7637.
PMID: 36496409
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

W Cheng et al. (2021-12-08), Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma Nature Communications, 2021-112(1):7142.
PMID: 34880251
Species: Human
Sample Types: Protein
Applications: Western Blot

B Nam et al. (2020-12-21), TGF&beta1 Suppressed Matrix Mineralization of Osteoblasts Differentiation by Regulating SMURF1-C/EBP&beta-DKK1 Axis International Journal of Molecular Sciences, 2020-121(24).
PMID: 33371439
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

B Wang et al. (2019-02-07), POH1 contributes to hyperactivation of TGF-? signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-? receptors and caveolin-1 EBioMedicine, 2019-00(0).
PMID: 30745168
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Ying Chen et al. (2017-08-17), MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells. British Journal of Cancer, 2017-00(0):1532-1827.
PMID: 28817834
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Gore B et al. (2014-06-23), Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension. PLoS ONE, 2014-09(6):e100310.
PMID: 24956016
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Lei Z et al. (2014-02-20), MiR-142-3p represses TGF-beta-induced growth inhibition through repression of TGFbetaR1 in non-small cell lung cancer. FASEB J, 2014-028(6):2696-704.
PMID: 24558198
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,ICC

Yao Y et al. (2006-09-27), Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells. Cardiovasc. Res., 2006-074(2):279-89.
PMID: 17074310
Species: Bovine
Sample Types: Tissue Homogenates
Applications: Western Blot

Haynes LP et al. (2006-03-01), Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta. Proteomics, 2006-06(6):1822-32.
PMID: 16470652

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Customer Reviews for Human TGF-beta RI/ALK‑5 Antibody (2)

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