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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 336304

Product Summary for Human Syndecan‑4 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Syndecan-4
Ser20-Glu145
Accession # P31431

Specificity

Detects human Syndecan-4 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Syndecan‑4 Antibody

Detection of Syndecan-4 antibody in Human HeLa Cell Line antibody by Flow Cytometry.

Detection of Syndecan-4 in Human HeLa Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Syndecan-4 Monoclonal Antibody (Catalog # MAB29181, filled histogram) or Rat IgG2A isotype control antibody (Catalog # MAB006, open histogram) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Syndecan‑4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-4

Syndecan-4, previously known as Amphiglycan or Ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying Heparan Sulfate (HS) and Chondroitin Sulfate (CS) glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa sequence identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present FGF‑2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6‑8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).

References

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488. 
  2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
  3. David, G. et al. (1992) J. Cell Biol. 118:961. 
  4. Choi, S. et al. (2005) J. Biol. Chem. 280:42573. 
  5. Charnaux, N. et al. (2005) FEBS J. 272:1937.  
  6. Schmidt, A. et al., J. Biol. Chem. 280:34441.
  7. Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507.
  8. Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811.
  9. Ishiguro, K. et al. (2003) Glycoconj. J. 19:315.
  10. Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.

Alternate Names

Amphiglycan, Ryudocan, SDC4, Syndecan4

Entrez Gene IDs

6385 (Human); 20971 (Mouse)

Gene Symbol

SDC4

UniProt

Additional Syndecan-4 Products

Product Documents for Human Syndecan‑4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Syndecan‑4 Antibody

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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