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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 336304

Product Summary for Human Syndecan‑4 APC‑conjugated Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Syndecan-4
Ser20-Glu145
Accession # P31431

Specificity

Detects human Syndecan-4 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Syndecan‑4 APC‑conjugated Antibody

Detection of Syndecan-4 antibody in Activated Lymphocytes antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Syndecan‑4 in Activated Lymphocytes in Human PBMCs by Flow Cytometry.

Activated lymphocytes in human peripheral blood mononuclear cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Rat Anti-Human Syndecan-4 APC-conjugated Monoclonal Antibody (Catalog # FAB29181A) or (B) Rat IgG2AAllophycocyanin Isotype Control (IC006A). View our protocol for Staining Membrane-associated Proteins.

Detection of Syndecan‑4 in U87MG Human Cell Line by Flow Cytometry.

U87MG human glioblastoma cell line was stained with Rat Anti-Human Syndecan-4 APC-conjugated Monoclonal Antibody (Catalog # FAB29181A, filled histogram) or Rat IgG2A Allophycocyanin Isotype Control (IC006A ,open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Syndecan‑4 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Activated lymphocytes in human peripheral blood mononuclear cells and U87MG human glioblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using FAB29181A in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Syndecan-4

Syndecan-4, previously known as Amphiglycan or Ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying Heparan Sulfate (HS) and Chondroitin Sulfate (CS) glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa sequence identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present FGF‑2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6‑8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).

References

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488. 
  2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
  3. David, G. et al. (1992) J. Cell Biol. 118:961. 
  4. Choi, S. et al. (2005) J. Biol. Chem. 280:42573. 
  5. Charnaux, N. et al. (2005) FEBS J. 272:1937.  
  6. Schmidt, A. et al., J. Biol. Chem. 280:34441.
  7. Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507.
  8. Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811.
  9. Ishiguro, K. et al. (2003) Glycoconj. J. 19:315.
  10. Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.

Alternate Names

Amphiglycan, Ryudocan, SDC4, Syndecan4

Entrez Gene IDs

6385 (Human); 20971 (Mouse)

Gene Symbol

SDC4

UniProt

Additional Syndecan-4 Products

Product Documents for Human Syndecan‑4 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Syndecan‑4 APC‑conjugated Antibody

For research use only

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