Human Serpin E1/PAI-1 Antibody

Neutralization of Serpin E1/ PAI‑1 Activity by Human Serpin E1/PAI‑1 Antibody.

Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) activity is measured in the presence of Recombinant Human Serpin E1 (1.35 µg/mL, Catalog # 1786-PI) that has been preincubated with increasing concentrations of Goat Anti-Human Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1786). The ND₅₀ is typically 10 µg/mL.

Detection of Serpin E1/PAI-1 by Immunohistochemistry

The expression of PAI-1 and LRP1 in human ESCC tissues. A Immunohistochemical staining for PAI-1 in human ESCC tissues. Representative images are shown: PAI-1 low-intensity in the stroma (left) and PAI-1 high-intensity in the stroma (right). Scale bars = 100 μm. b High-power fields of immunohistochemical staining for PAI-1 in same cases as Fig. 6a. Scale bars = 20 μm. Arrow heads: vascular endothelial cell. c Immunohistochemical staining for LRP1 in cancer nest of human ESCC tissues. Representative images are shown: LRP1 low-intensity in cancer nest (left) and LRP1 high-intensity in cancer nest (right). Scale bars = 100 μm. d Immunohistochemical staining for LRP1 in the stroma of human ESCC tissues. Representative images are shown: LRP1 low-intensity in the stroma (left) and LRP1 high-intensity in the stroma (right). Scale bars = 20 μm. e Stromal cells, including CD204+ TAMs, expressed LRP1 in human ESCC tissue. Double immunofluorescence was performed using anti-LRP1 (green) and anti-CD204 (red) antibodies in formalin-fixed paraffin-embedded section of human ESCC tissue. LRP1 was expressed in both cancer cells and stromal cells, and some of the LRP1-positive stromal cells were CD204+ macrophages (arrow heads). Nuclei were stained with DAPI (blue). Scale bar = 20 μm. CA cancer nest. ST stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33311557), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Serpin E1/PAI-1 by Immunohistochemistry

The expression of PAI-1 and LRP1 in human ESCC tissues. A Immunohistochemical staining for PAI-1 in human ESCC tissues. Representative images are shown: PAI-1 low-intensity in the stroma (left) and PAI-1 high-intensity in the stroma (right). Scale bars = 100 μm. b High-power fields of immunohistochemical staining for PAI-1 in same cases as Fig. 6a. Scale bars = 20 μm. Arrow heads: vascular endothelial cell. c Immunohistochemical staining for LRP1 in cancer nest of human ESCC tissues. Representative images are shown: LRP1 low-intensity in cancer nest (left) and LRP1 high-intensity in cancer nest (right). Scale bars = 100 μm. d Immunohistochemical staining for LRP1 in the stroma of human ESCC tissues. Representative images are shown: LRP1 low-intensity in the stroma (left) and LRP1 high-intensity in the stroma (right). Scale bars = 20 μm. e Stromal cells, including CD204+ TAMs, expressed LRP1 in human ESCC tissue. Double immunofluorescence was performed using anti-LRP1 (green) and anti-CD204 (red) antibodies in formalin-fixed paraffin-embedded section of human ESCC tissue. LRP1 was expressed in both cancer cells and stromal cells, and some of the LRP1-positive stromal cells were CD204+ macrophages (arrow heads). Nuclei were stained with DAPI (blue). Scale bar = 20 μm. CA cancer nest. ST stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33311557), licensed under a CC-BY license. Not internally tested by R&D Systems.

Human Serpin E1 / PAI-1 ELISA Standard Curve

Recombinant Human Serpin E1/PAI-1 (Catalog # 1786-PI) was serially diluted and captured by Mouse Anti-Human Serpin E1/PAI-1 Monoclonal Antibody (Catalog # MAB1786) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1786) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)